Recombinant Mouse IL-33 R

Recombinant Mouse IL-33 Receptor (IL-33 R) is used in research applications to study the molecular mechanisms of IL-33 signaling, dissect receptor-ligand interactions, and modulate immune responses in mouse models. Its use enables precise investigation of the IL-33/ST2 axis, which is implicated in inflammation, tissue repair, allergy, and cancer immunology.

Key scientific applications and rationale include:

• Mechanistic Studies: Recombinant IL-33 R allows researchers to analyze how IL-33 binds and activates its receptor, ST2, and downstream signaling pathways. This is essential for understanding the role of IL-33 in immune cell activation, cytokine production, and tissue-specific responses.

• Blocking or Modulating IL-33 Signaling: By using recombinant IL-33 R as a decoy or competitor, researchers can inhibit endogenous IL-33 activity, helping to clarify its contribution to disease models such as fibrosis, allergy, autoimmunity, and cancer.

• Therapeutic Target Validation: The IL-33/ST2 axis is a promising therapeutic target for conditions like allergic diseases, tissue injury, and cancer. Recombinant IL-33 R is used to validate the effects of receptor blockade or enhancement in preclinical studies.

• Functional Assays: Recombinant IL-33 R is employed in bioassays to measure IL-33 activity, receptor binding affinity, and downstream effects on immune cells (e.g., Tregs, ILC2s, mast cells).

• Disease Modeling: In mouse models, manipulating IL-33 signaling via recombinant receptor helps elucidate its role in processes such as wound healing, fibrosis, neuroinflammation, and tumor immunity.

Best Practices:

• Use recombinant IL-33 R in controlled in vitro or in vivo experiments to assess its impact on cytokine production, immune cell polarization, and tissue pathology.
• Combine with recombinant IL-33 ligand or genetic models to dissect specific pathway contributions.
• Validate specificity and activity using appropriate controls and dose-response studies.

Summary:
Using recombinant mouse IL-33 R in research provides a powerful tool for dissecting IL-33 biology, validating therapeutic strategies, and modeling disease mechanisms in mice, especially where modulation of the IL-33/ST2 axis is central to the experimental question.

Yes, recombinant Mouse IL-33 R (ST2/IL-33R) can be used as a standard for quantification or calibration in ELISA assays, provided the assay is specifically designed to measure ST2/IL-33R and the recombinant protein matches the form detected by your antibodies.

For ELISA quantification, the standard must be the same analyte as the target measured in your samples. If your assay is designed to detect mouse IL-33 R (ST2/IL-33R), recombinant mouse ST2/IL-33R is appropriate as a standard. Commercial ELISA kits for mouse ST2/IL-33R use recombinant mouse ST2/IL-33R as their calibration standard, and protocols typically include serial dilutions of the recombinant protein to generate a standard curve for quantification.

Key considerations:

• The recombinant standard should be of the same isoform and post-translational modification state as the native protein in your samples, as differences may affect antibody recognition and quantification accuracy.
• The standard curve must be prepared freshly for each assay, and technical errors in its preparation can compromise quantification.
• Always refer to your ELISA kit's instructions for recommended reconstitution, dilution, and standard curve preparation, as these are optimized for the specific antibodies and detection system used.

If your assay is designed to measure IL-33 (the ligand), not the receptor (ST2/IL-33R):

• You must use recombinant mouse IL-33 as the standard, not the receptor. Using the wrong standard will yield inaccurate results.

Summary Table:

Best Practices:

• Confirm the specificity of your antibodies for the recombinant standard.
• Validate parallelism between standard curves and sample dilution curves to ensure accurate quantification.
• Use carrier-free recombinant protein if required for ELISA, as carriers (e.g., BSA) may interfere with detection.

If you clarify whether your assay targets IL-33 or IL-33R/ST2, more tailored guidance can be provided.

Recombinant Mouse IL-33 Receptor (IL-33 R) has been validated in published research primarily for applications involving the study of immune signaling, inflammation, and cell activation in mouse models.

Key validated applications include:

• Bioassays: Used to assess the functional activity of IL-33 signaling, including stimulation of Th2 lymphocytes, induction of cytokine secretion (e.g., IL-5, IL-13), and activation of various immune cells such as macrophages, neutrophils, B cells, eosinophils, basophils, and mast cells.
• In Vivo Studies: Employed in mouse models to investigate the physiological and pathological roles of IL-33 signaling, such as modulation of immune responses, tumorigenesis, allergic inflammation, and tissue regeneration.
• Cell Culture: Utilized in vitro to stimulate primary cells or cell lines, enabling analysis of downstream signaling pathways, gene expression, and cytokine production.
• ELISA Standard: Used as a reference standard for quantifying IL-33 or its receptor in immunoassays.
• Angiogenesis and Endothelial Activation: Applied to study vascular responses, including angiogenesis and endothelial cell activation in response to IL-33 signaling.
• Neurobiology and Bone Research: Used in models assessing memory, learning, and bone health, demonstrating effects on neural function and osteogenesis.

Additional validated research contexts:

• Tumor Immunology: Investigating the role of IL-33/IL-33R signaling in anti-tumor immunity, including recruitment and activation of eosinophils and regulatory T cells.
• Allergic and Type 2 Immunity: Studying mechanisms of allergic airway disease and type 2 immune responses, particularly in lung and mucosal tissues.
• Tissue Repair and Regeneration: Exploring the involvement of IL-33R in wound healing and tendon regeneration.

Summary Table of Validated Applications

These applications are supported by multiple peer-reviewed studies and reviews, demonstrating the broad utility of recombinant mouse IL-33 R in immunology, cell biology, and disease modeling.

To reconstitute and prepare Recombinant Mouse IL-33 R (also known as ST2 or IL-33 Receptor) protein for cell culture experiments, follow these general guidelines based on standard protocols for recombinant receptor proteins such as soluble ST2/IL-33R:

Reconstitution

1. Centrifugation: Briefly centrifuge the lyophilized vial before opening to ensure all contents are at the bottom.
2. Reconstitution Buffer: Reconstitute the lyophilized protein in sterile phosphate-buffered saline (PBS) or a buffer compatible with your cell culture system (e.g., serum-free medium). Avoid buffers containing reducing agents unless specified.
3. Concentration: For most recombinant soluble receptor proteins (such as Mouse IL-33 R/ST2), a typical reconstitution concentration is 500 µg/mL in PBS. Adjust according to the manufacturer’s instructions or experimental needs.
4. Mixing: Gently swirl or pipette up and down to dissolve the protein completely. Avoid vigorous vortexing to prevent denaturation.

Preparation for Cell Culture

1. Aliquoting: After reconstitution, aliquot the protein into small, single-use volumes to minimize freeze-thaw cycles.
2. Storage: Store aliquots at ≤ –20°C (preferably –80°C for long-term storage). Avoid repeated freeze-thaw cycles.
3. Working Dilution: Dilute the reconstituted protein in cell culture medium (with or without serum, depending on your assay) immediately before use. Typical working concentrations for soluble receptors in cell culture range from 0.1–10 µg/mL, but optimize based on your experimental setup and cell type.
4. Sterility: Ensure all steps are performed under sterile conditions to prevent contamination.

Additional Notes

• Soluble Receptor Function: Soluble IL-33 R (sST2) acts as a decoy receptor, neutralizing IL-33 activity. It is commonly used to inhibit IL-33 signaling in cell culture experiments.
• Controls: Include appropriate controls (e.g., vehicle-only or irrelevant protein) to confirm specificity.

Always refer to the specific product datasheet for exact reconstitution and storage instructions, as formulations may vary between suppliers.