Recombinant Mouse OSM Rβ is used in research applications to specifically study the signaling pathways and biological functions mediated by the Oncostatin M receptor beta (OSMRβ), which is a critical component of the OSM receptor complex involved in cytokine signaling, immune regulation, and disease models.

Key scientific reasons to use Recombinant Mouse OSM Rβ:

• Specificity for OSM Signaling: OSMRβ is a unique subunit of the OSM receptor complex, allowing researchers to dissect OSM-specific signaling events, distinct from other IL-6 family cytokines.
• Modeling Disease Mechanisms: OSMRβ-mediated pathways are implicated in inflammation, hematopoiesis, skin diseases (such as atopic dermatitis), and cancer metastasis. Recombinant OSM Rβ enables controlled in vitro and in vivo studies of these processes.
• Functional Assays: Recombinant OSM Rβ can be used in cell-based assays to evaluate ligand binding, receptor activation, downstream signaling (e.g., STAT3 phosphorylation), and cellular responses such as proliferation, differentiation, or cytokine production.
• Therapeutic Target Validation: By using recombinant OSM Rβ, researchers can screen for inhibitors or antibodies that block OSMRβ signaling, which is relevant for developing therapies for inflammatory and autoimmune diseases.
• Multiplex Assays: Recombinant OSM Rβ is compatible with multiplex biomarker assays, enabling simultaneous quantification of OSMRβ and related cytokines in complex biological samples.

Best practices and applications:

• Use recombinant OSM Rβ in cell culture experiments to study receptor-ligand interactions and downstream effects in mouse cells expressing OSMRβ.
• Employ recombinant OSM Rβ in in vivo models to investigate its role in disease progression, immune modulation, and tissue-specific responses.
• Integrate recombinant OSM Rβ in bioassays for screening potential therapeutic compounds or antibodies targeting the OSMRβ pathway.

Technical advantages:

• Recombinant proteins offer high purity, batch-to-batch consistency, and defined activity, which are essential for reproducible and interpretable experimental results.
• Recombinant OSM Rβ enables precise control over experimental conditions, facilitating mechanistic studies and quantitative analyses.

In summary, Recombinant Mouse OSM Rβ is a valuable tool for elucidating OSMRβ-dependent signaling, modeling disease mechanisms, and validating therapeutic strategies in mouse systems, with broad applications in immunology, cell biology, and translational research.

Recombinant Mouse OSM Rβ (Oncostatin M Receptor beta) is not suitable as a standard for quantification or calibration in ELISA assays designed to measure Oncostatin M (OSM) protein levels. ELISA standards must match the analyte being quantified—in this case, OSM, not its receptor.

Key points:

• ELISA standards should be a purified or recombinant form of the target analyte (here, mouse OSM), not a receptor or binding partner.
• Recombinant mouse OSM protein is commonly used as a standard in OSM ELISAs, as it provides a reliable and quantifiable reference for generating standard curves.
• Using OSM Rβ (the receptor) as a standard would not provide meaningful calibration for OSM quantification, since the antibody pairs in OSM ELISAs are specific for OSM, not its receptor.
• ELISA kits and protocols specifically state the use of recombinant OSM as the standard, and not the receptor or other related proteins.

Best practice:
Use recombinant mouse OSM protein, ideally matched to the form detected by your assay (e.g., mature OSM), for standard curve preparation in OSM ELISAs. Only use OSM Rβ as a standard if your assay is specifically designed to detect OSM Rβ, which is a different application.

If you need to quantify OSM, do not use OSM Rβ as a standard. Always match your standard to the analyte your ELISA is designed to detect.

Applications of Recombinant Mouse OSM Rβ in Published Research

Recombinant mouse OSM receptor-beta (OSMRβ) has been validated across several important research applications, primarily centered on understanding the OSM-OSMRβ signaling axis in various physiological and pathological contexts.

Wound Healing and Tissue Repair

One of the most extensively studied applications involves wound healing, particularly in diabetic contexts. Recombinant OSM administered topically has been validated to enhance wound closure by promoting fibroblast expression of tissue inhibitor of metalloproteinases-1 (TIMP-1) and hepatocyte growth factor (HGF). The OSM-OSMRβ axis has been shown to accelerate granulation tissue formation, increase myofibroblast proliferation, and promote neovascularization at wound sites. In diabetic mouse models, exogenous OSM treatment significantly improved delayed wound healing compared to control groups.

Immune Cell Differentiation and Function

Recombinant OSM has been validated in bioassay applications demonstrating its role in immune cell maturation and polarization. Research has shown that OSM induces dendritic cell maturation and promotes Th1 polarization. Additionally, OSM enhances chemokine ligand 21 (CCL21) expression by microvascular endothelial cells, increasing the efficiency of dendritic cell trafficking to lymph nodes.

Hematopoietic Stem Cell Signaling

The OSM-OSMRβ signaling pathway has been validated in studies examining hematopoietic stem cell (HSC) biology. Recombinant murine OSM (at concentrations of 500 ng/ml) has been used in RNA-sequencing studies to investigate OSMRβ-dependent gene expression changes in adult mouse HSCs.

Inflammatory and Dermatological Disease Models

Recombinant OSM and OSMRβ-targeting approaches have been validated in atopic dermatitis models. Treatment with monoclonal antibodies against OSMRβ has been shown to reduce skin severity scores in atopic dermatitis model mice, suggesting that OSM signaling blockade can ameliorate inflammatory skin lesions.

Cardiovascular Applications

OSM receptor signaling has been investigated as a therapeutic target in heart failure prevention, indicating validation of OSMRβ in cardiovascular disease research contexts.

To reconstitute and prepare Recombinant Mouse OSM Rβ protein for cell culture experiments, dissolve the lyophilized protein in sterile water or PBS at a concentration between 25–100 μg/mL, optionally including 0.1% carrier protein (such as BSA) to stabilize the protein and prevent adsorption to surfaces.

Step-by-step protocol:

• Centrifuge the vial briefly to collect the lyophilized powder at the bottom before opening.
• Add sterile water or PBS:
  • For most applications, reconstitute at 25–100 μg/mL in sterile PBS or water.
  • If recommended, include 0.1% BSA (bovine serum albumin) in PBS to enhance stability, especially for low protein concentrations or long-term storage.
• Gently mix by swirling or inverting; avoid vigorous shaking to prevent foaming and protein denaturation.
• Allow to dissolve for 15–30 minutes at room temperature with gentle agitation.
• Aliquot and store:
  • For short-term use (up to one week), store at 2–8°C.
  • For long-term storage, aliquot and freeze at –20°C or –80°C, avoiding repeated freeze-thaw cycles.
• Dilute to working concentration in cell culture medium immediately before use. Ensure the final buffer is compatible with your assay and cell type.

Additional notes:

• Always consult the specific product datasheet for any unique requirements regarding buffer composition or reconstitution concentration.
• Carrier proteins (e.g., BSA) are recommended for low concentrations or when adsorption to plasticware is a concern.
• Avoid reconstituting below 25 μg/mL unless specifically validated, as lower concentrations may reduce stability.

Summary Table:

This protocol ensures optimal solubility, stability, and biological activity of recombinant Mouse OSM Rβ protein for cell culture experiments.