Streptavidin – Alkaline Phosphatase

Streptavidin – Alkaline Phosphatase

Product No.: S208

[product_table name="All Top" skus="S208"]

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Product Type
Avidin - Biotin

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Background

Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (Kd~10 -15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Protein Details

Format
Alkaline Phosphatase (Alk Phos)
Product Concentration
1.0 mg/ml
Formulation
Streptavidin-Alk. Phos. is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.
Working Concentration
Western blotting: 0.1 - 1.0 µg/ml
ELISA: 1 - 2 µg/ml
Immunohistochemistry: 1 - 2 µg/ml
Western blotting and ELISA Chemiluminescent: 0.1 - 0.05 µg/ml

However, each investigator should determine their own optimal working dilution for each specific research application.
Storage and Stability
This Alkaline Phosphatase conjugate is stable for ~6 weeks when stored at 2-8°C. Do not freeze. For extended storage, add an equal volume of ACS grade or better glycerol for a final concentration of 50%, then store at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.