UltraAvidin™- Alkaline Phosphatase

Pricing & Details

  
Prod No.
Size
Price
Avail.
Qty
Add to cart
A108-1.0 mg
1.0 mg
$150.00
In stock
A108-2.0 mg
2.0 mg
$225.00
In stock
  

Antibody Details

Product Details

Product Concentration
1.0 mg/ml
Formulation
UltraAvidin™-Alkaline Phosphatase (Alk. Phos.) is supplied in 0.05M Tris pH 8.0, containing .001M MgCl2</sub), .0001M ZnCl2, 1.0% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This Alkaline Phosphatase conjugate is stable when stored at 2-8°C. Do not freeze
Country of Origin
USA
See directions on lot specific datasheets, as information may periodically change.
Working Concentration
UltraAvidin™-Alkaline Phosphatase can be used to detect biotinylated biological probes such as antibodies or cytokines in a variety of applications such as protein blotting, immunohistochemistry or ELISA.

The suggested working concentration for ELISA, immunohistochemistry and western blotting is approximately 0.1 - 2.0 µg/ml. However, each investigator should determine their own optimal working dilution for each specific research application.
Isoelectric Point
pI= 6.3 +/- 0.3
Specific Activity
11-17 µg biotin bound/mg UltraAvidin™
Each investigator should determine their own optimal working dilution for specific applications.

Description

Background
UltraAvidin™ is Leinco Technologies' uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000, there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin™ from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin™ has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

Recent studies have identified a universal recognition sequence in streptavidin which is similar to that found in several adhesion receptors.¹ Strong interactions have been shown with streptavidin and cell surface molecules. This universal recognition sequence is not present in UltraAvidin™. UltraAvidin™ may not significantly increase sensitivity in all research systems but it will assure specificity.

UltraAvidin™ has been covalently conjugated to Alkaline Phosphatase from calf mucosa. The conjugate is then purified chromatographically to remove unconjugated enzyme and UltraAvidin™.

Antigen Details

References & Citations

1. Wilchek, M. et al.(1990) Biochem Biophys Res Commun. 1990 170(3):1236-41.
  
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.