BriClone and BriClone-SF
The Gold Standard in Hybridoma Cloning Supplements.
BriClone is a specialized hybridoma cloning supplement designed to enhance the efficiency of post-fusion stages of hybridoma production and cell cloning. This crucial process generates monoclonal antibodies vital for research and diagnostics. By using BriClone as a post-fusion cloning medium, you can streamline your antibody production workflow optimization and ensure higher success rates in stable cell line generation for mAbs.
Why Choose BriClone as Your Hybridoma Growth Supplement?
Integrating BriClone into your cell line development reagents toolkit allows you to achieve:
- Higher Cloning Efficiency: BriClone significantly increases the number of viable hybridoma colonies, leading to greater antibody production.
- Reduced Feeder Cell Dependence: It serves as a superior feeder cell replacement for hybridomas, minimizing or eliminating the need for feeder cells, simplifying your workflow, and reducing costs.
- Consistent Performance: BriClone helps in overcoming batch variation in feeder cells, ensuring reliable and reproducible results across experiments.
- Improved Productivity: Achieve higher yields of monoclonal antibodies, accelerating your research and development timelines.
Streamlined Feeder-Free Hybridoma Culture
BriClone is a conditioned medium for hybridoma growth derived from a human cell line. It is supplied sterile and frozen in convenient 100ml bottles, ready to be incorporated into your limiting dilution cloning supplement protocols at a recommended concentration. It is a cost-effective solution that simplifies improving hybridoma fusion efficiency, making it the preferred choice for researchers worldwide
| Product Name | Catalog Number | Size | Key Application |
|---|---|---|---|
| BriClone | BRI-10000 | 100 ml | Hybridoma single cell cloning medium |
| BriClone-SF | BR-002 | 100 ml | Serum-free hybridoma growth supplement |
Figure 1. Comparison of hybridoma cloning efficiency using Briclone and HyMax supplements.
Fused naïve mouse splenocytes were seeded at 1 x 10^5 cells/well in 96-well plates (n = 5 plates per condition, 90 wells/plate) and maintained under HAT selection. Data points represent the mean percentage of growth-positive wells per plate recorded at 7, 14, 16, and 21 days post-fusion. Briclone at both 1% and 5% concentrations demonstrated growth kinetics and final cloning efficiencies comparable to those of HyMax at a 1% concentration.
