BriClone Hybridoma Cloning Medium (BRI10000)
Data
To demonstrate the stability of BriClone, various simulated conditions were assessed mimicking different temperature exposures. Post-fusion hybridoma production was assessed using BriClone under different conditions (overnight at room temperature) as a positive control, as well as one week stored at 4°C. Fusion efficiency is expressed as a percentage of wells in 48-well plates with positive hybridoma clones following fusion. No notable difference was observed in hybridoma yield between use of BriClone thawed overnight at room temperature or after being stored for one week at 4˚C. Stability of BriClone is therefore not significantly affected following exposure to extended ambient temperatures as may occasionally be observed in transport of the product on ice packs. Negative control was performed without any supplement.
Many commercial additives are available for improving the improving the efficiency of cell cloning at the post-fusion stages of hybridoma production. In the following experiment, BriClone was compared to two competitors as a supplement for post-fusion hybridoma production by assessing fusion efficiency. Fusion efficiency is expressed as a percentage of wells in 48-well plates with positive hybridoma clones following fusion, relative to BriClone positive control (thawed overnight). All supplements used at their recommended concentrations. BriClone increased the number of positive wells identified with hybridoma clones, when compared to two separate competitors. Negative control indicates no supplement added. - -
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Antibody DetailsProduct DetailsProduct Concentration 20X Solution Formulation Dulbeccos Modified Eagle medium (DMEM): Non Animal Source<br><br>
Foetal Bovine Serum: origin United States of America, certified free
of viruses State of Matter Liquid Storage and Handling Store at -20°C. Avoid repeated freeze/thaw cycles. Stable for short periods at +4°C Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Thaw and add to the hybridoma cloning medium as a 5% v/v supplement. • Hybridoma growth post-fusion (refer to your own protocol) • Hybridoma Cloning (refer to your own protocol) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity BriClone Hybridoma Cloning Medium Background BriClone is an additive for the cloning medium used in the post-fusion stages of hybridoma production and for improving the efficiency of hybridoma cells cloning. BriClone completely replaces the need for feeder cells. The use of feeder cells to support the outgrowth of hybridomas has many disadvantages, like batch-to-batch variation of the feeder cells and competition between feeder cells and freshly-fused hybridomas for nutrients in the culture medium. BriClone has been specially developed to overcome these common problems in the post-fusion period. References & CitationsPMC7873262,PMC7239657,PMC6803335,PMC6816435,PMC6719973,PMC5017023,PMC2812581,PMC2896538,PMC4617144,PMC3944398,PMC9030397,PMC8470961,PMC8104110,PMC7279471,PMC7096428,PMC5992998,PMC5986985,PMC5857169,PMC4921032,PMC4591809,PMC4135088,PMC1858089,PMC1602225 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
