BriClone Hybridoma Cloning Medium
BriClone Hybridoma Cloning Medium
Product No.: BRI10000
Data
To demonstrate the stability of BriClone, various simulated conditions were assessed mimicking different temperature exposures. Post-fusion hybridoma production was assessed using BriClone under different conditions (overnight at room temperature) as a positive control, as well as one week stored at 4°C. Fusion efficiency is expressed as a percentage of wells in 48-well plates with positive hybridoma clones following fusion. No notable difference was observed in hybridoma yield between use of BriClone thawed overnight at room temperature or after being stored for one week at 4˚C. Stability of BriClone is therefore not significantly affected following exposure to extended ambient temperatures as may occasionally be observed in transport of the product on ice packs. Negative control was performed without any supplement.
Many commercial additives are available for improving the improving the efficiency of cell cloning at the post-fusion stages of hybridoma production. In the following experiment, BriClone was compared to two competitors as a supplement for post-fusion hybridoma production by assessing fusion efficiency. Fusion efficiency is expressed as a percentage of wells in 48-well plates with positive hybridoma clones following fusion, relative to BriClone positive control (thawed overnight). All supplements used at their recommended concentrations. BriClone increased the number of positive wells identified with hybridoma clones, when compared to two separate competitors. Negative control indicates no supplement added.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
