BriClone SF (Serum Free) Hybridoma Cloning Medium 20x (BR-002)
Data
Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone (red) compared to Bri-Clone-SF (brown) and a negative control (blue).
BriClone-SF is stable for at least 9 months when stored at -20˚C. Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone-SF stored at 4˚C for 2 weeks, Room Temperature for 1 week and -20˚C for 9 months compared to a negative control (blue).
BriClone-SF has equivalent fusion efficiency activity when compared to two leading competitor products when used at the manufacturer’s recommended concentrations. Percentage of fusion efficiency from freshly fused hybridomas cultured using BriClone-SF (orange) compared to Competitor A (light grey) and competitor B (dark grey) products and a negative control (blue). - -
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Antibody DetailsProduct DetailsProduct Concentration 20X Solution Formulation Dulbeccos Modified Eagle medium (DMEM): Non Animal Source<br><br>
Foetal Bovine Serum: origin United States of America, certified free
of viruses State of Matter Liquid Storage and Handling Store at -20°C. Avoid repeated freeze/thaw cycles. Stable for short periods at +4°C Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Thaw and add to the hybridoma cloning medium as a 5% v/v supplement. • Hybridoma growth post-fusion (refer to your own protocol) • Hybridoma Cloning (refer to your own protocol) Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity BriClone SF Hybridoma Cloning Medium Background BriClone is produced by exposing basal culture medium with a low percentage of fetal calf
serum to a human cancer cell line, which secretes relevant molecules into the medium,
resulting in a product consistently supportive of the fusion and cloning stages of
hybridoma production. Some researchers require a product which is serum-free and has had
no contact with human or animal cells. We have, therefore, designed BriClone-SF which has
the same basal medium and biological activities as BriClone but has no added serum or
other substances of human or animal origin and has not been exposed to cells. BriClone-SF
contains added recombinant human proteins produced in bacterial or yeast host cells.
References & CitationsPMC7873262,PMC7239657,PMC6803335,PMC6816435,PMC6719973,PMC5017023,PMC2812581,PMC2896538,PMC4617144,PMC3944398,PMC9030397,PMC8470961,PMC8104110,PMC7279471,PMC7096428,PMC5992998,PMC5986985,PMC5857169,PMC4921032,PMC4591809,PMC4135088,PMC1858089,PMC1602225 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
