Choose a CTLA-4 Antibody For Your Research

CTLA-4 Introduction

Cytotoxic T-lymphocyte–associated antigen 4 (CTLA-4) is a critical immune checkpoint receptor predominantly found on T cells. CTLA-4 plays a central role in maintaining immune system balance by acting as a negative regulator of T-cell activation. It competes with the costimulatory receptor CD28 for binding to the ligands CD80 (B7-1) and CD86 (B7-2) on antigen-presenting cells (APCs). Unlike CD28, which promotes T-cell activation, CTLA-4 delivers inhibitory signals that suppress T-cell responses and limit immune activation. CTLA-4 is highly expressed on regulatory T cells (Tregs), where it contributes to immunosuppression by blocking the activation of effector T cells. This mechanism helps prevent autoimmunity but can also hinder anti-tumor immunity by allowing cancer cells to evade immune detection.

CTLA4 antibodies—also known as anti-CTLA4 antibodies—are designed to block the interaction between CTLA-4 and its ligands (CD80/CD86). By inhibiting CTLA-4 signaling, these immune checkpoint inhibitors restore T-cell activation and enhance the body’s ability to mount effective anti-tumor immune responses. CTLA4-blocking antibodies, such as ipilimumab, have shown significant clinical success in cancer immunotherapy, particularly for melanoma and other solid tumors. The mechanism of action of CTLA4 antibodies involves releasing the brakes on the immune system, allowing cytotoxic T cells to recognize and destroy tumor cells more effectively. This checkpoint blockade strategy is a cornerstone of modern immuno-oncology.

Discover Leinco’s Anti-Mouse CTLA-4 Antibody Clones

Leinco Technologies provides researchers with a comprehensive selection of anti-mouse CTLA-4 antibodies derived from three unique hybridoma clones: 9H10, 9D9, and UC10-4F10-11. These anti-mouse CTLA-4 (CD152) antibodies are essential tools for diverse research applications, offering distinct mechanisms of action and documented efficacies.

Explore Our Anti-Mouse CTLA-4 Antibody Clones:

• Anti-mouse CTLA-4 (CD152) Clone 9D9: This mouse monoclonal antibody (Quezada et al. 2006) is a powerful tool for in vivo studies. It functions by depleting intra-tumoral regulatory T cells (Tregs) and is also proven to neutralize CTLA-4 in vivo.

• Anti-mouse CTLA-4 (CD152) Clone 9H10: A Syrian hamster IgG (Krummel and Allison 1995), 9H10 promotes T cell co-stimulation. It achieves this by effectively blocking CTLA-4 from binding to its ligands, thereby allowing CD28 binding. This anti-mouse CTLA-4 antibody is reported to neutralize CTLA-4 both in vitro and in vivo. Notably, 9H10 also depletes intra-tumoral Tregs and demonstrated slightly stronger depletion efficacy than 9D9 in a mouse melanoma model (Simpson et al., 2013).

• Anti-mouse CTLA-4 (CD152) Clone UC10-4F10-11: This Armenian hamster IgG (Walunas et al. 1994) is an excellent choice for neutralizing CTLA-4 in vitro and in vivo.

Versatile Applications for Your Research:

All three anti-mouse CTLA-4 antibodies are suitable for detecting murine CTLA-4 via Western blot. Additionally, the UC10-4F10-11 clone can be utilized for flow cytometry, expanding its utility in your experiments. Accordingly, the primary differences between various CTLA4 antibodies are defined by their CTLA-4 blocking efficiency, their published or reported research and clinical applications, as well as their host species and antibody isotypes.

Which CTLA-4 Clone Is Best For My Research?

Choosing the right anti-mouse CTLA-4 antibody from Leinco Technologies is crucial for the success of your research. We recommend performing a thorough literature search to identify published studies that align with your specific experimental model or approach.

You’ll often find that certain anti-mouse CTLA-4 antibody clones are more frequently utilized for particular mouse models and experimental setups due to their unique characteristics and documented efficacy. By reviewing existing publications, you can gain valuable insights into which Leinco Technologies clone might be best suited for your research, ensuring optimal results.