Anti-Mouse CTLA-4 (Clone 9H10) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C2841
Clone
9H10
Protein
CLTA-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56
Isotype
Syrian Hamster
IgG
Applications
B
,
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
C2841-1.0 mg
1.0 mg
$225.00
$175.50
In stock
Max:
Min: 1
Step: 1
C2841-5.0 mg
5.0 mg
$445.00
$347.10
In stock
Max:
Min: 1
Step: 1
C2841-25 mg
25 mg
$1,285.00
$1,002.30
In stock
Max:
Min: 1
Step: 1
C2841-50 mg
50 mg
$2,045.00
$1,595.10
In stock
Max:
Min: 1
Step: 1
C2841-100 mg
100 mg
$2,800.00
$2,184.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Data

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Syrian Hamster
Immunogen
Mouse CTLA-4-human IgG1 fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Mouse CLTA-4 (Clone 9H10) recognizes an epitope on Mouse CD152. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody was also pathogen tested and third-party certified by IDEXX BioReseach to meet the lowest mycoplasma specification and free of any viral pathogens of concern.
Antigen Distribution
Activated T cells
Background
CTLA4 (Cytotoxic T-Lymphocyte Antigen 4) also known as CD152, is a protein which is expressed on the surface of Helper T cells and plays an important regulatory role in the immune system.1 CTLA4 is a member of the immunoglobulin superfamily, expressed on the surface of Helper T cells. CTLA4 transmits an inhibitory signal to T cells.2,3 CTLA4 is potentially therapeutic in autoimmune diseases4, such as rheumatoid arthritis, HIV, autoimmune thyroid disease, multiple sclerosis and may also be useful during organ transplantation and cancer treatment. The 9H10 antibody has been shown to promote T cell co-stimulation by blocking CTLA-4 binding to the B7 co-receptors, allowing for CD28 binding.

Antigen Details

Protein
Ligand/Receptor
CD80 (B7.1), CD86 (B7.2)
Function
Negative regulator of T cell activation
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. Allison JP, et al. 1995. Science 270:932.
General Western Blot Protocol

Formats Available