Anti-Mouse CTLA-4 (Clone 9H10) – Purified in vivo GOLD™ Functional Grade

Anti-Mouse CTLA-4 (Clone 9H10) – Purified in vivo GOLD™ Functional Grade

Product No.: C1614

[product_table name="All Top" skus="C1614"]

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Clone
9H10
Target
CTLA-4
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56
Isotype
IgG
Applications
B
,
in vivo
,
WB

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Product Size

Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Syrian Hamster
Recommended Isotype Controls
Syrian Hamster IgG
Immunogen
Mouse CTLA-4-human IgG1 fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 9H10 recognizes an epitope on mouse CTLA-4.
Antigen Distribution
CTLA-4 is expressed on activated T and B lymphocytes.
Background
CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity.

Antigen Details

Ligand/Receptor
CD80 (B7-1), CD86 (B7-2)
Function
Negative regulator of T cell activation
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1.) Wurster S. et al. (2020) The Journal of Infectious Diseases 222(6):1989–994 Journal Link
B
in vivo Protocol
General Western Blot Protocol
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.