Goat Anti-Syrian Hamster IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Rb., Bv., Hs.) – Biotin

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
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S225-1.0 ml
1.0 ml
$193.00
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Antibody Details

Product Details

Reactivity Species
Syrian Hamster
Host Species
Goat
Immunogen
Purified Syrian Hamster IgG (H&L) (>98%)
Product Concentration
1.3 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Country of Origin
USA
Applications and Recommended Usage
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Working Concentration
It is recommended that working dilutions be prepared fresh daily.

ELISA: 1:20,000 - 1:40,000
IHC: 1:500 - 1:5,000
FC: 1:200 - 1:1,000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Goat Anti-Syrian Hamster IgG (H&L) recognizes Hamster IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Protein
IgG (H&L)

References & Citations

Products are for research use only. Not for use in diagnostic or therapeutic procedures.