Goat Anti-Syrian Hamster IgG (H&L) (Adsorbed Against: Ms., Hu., Rt., Rb., Bv., Hs.) – Biotin

Pricing & Details

  
Product Type
Secondary Antibody
Prod No.
Size
Price
Avail.
Qty
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S225-1.0 ml
1.0 ml
$193.00
In stock
  

Antibody Details

Reactivity Species
Syrian Hamster
Host Species
Goat
Immunogen
Purified Syrian Hamster IgG (H&L) (>98%)
Product Concentration
1.3 mg/ml
Formulation
This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Biotin conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
Expiration Date
One year from date of reconstitution
Working Concentration
It is recommended that working dilutions be prepared fresh daily.

ELISA: 1:20,000 - 1:40,000
IHC: 1:500 - 1:5,000
FC: 1:200 - 1:1,000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 5 µg/ml (100 µl/well) µg/ml.

IHC (NBF/Par.): This antibody may be used to stain nuclear membranes in cells, tissue preparations or subcellular fractions. The antibody works well with cells and tissue sections fixed with 2% paraformaldehyde and requires no dilution. Tonsil is the recommended positive control tissue.

Flow Cytometry: PN:A106

Western Blotting: To detect Human CASP8 by Western blot analysis, this antibody can be used at a concentration of 1.0-2.0 µg/ml (1:100 Dilution). When used in conjunction with compatible second step reagents such as PN:M114 and a chromogenic substrate such as PN:T343, the detection limit for Human CASP8 is 1.0 ng/lane under either reducing or non-reducing conditions.
Specificity
Goat Anti-Syrian Hamster IgG (H&L) recognizes Hamster IgG (H&L). This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.

Antigen Details

Protein Family
IgG (H&L)
Products are for research use only. Not for use in diagnostic or therapeutic procedures.