UltraAvidin™- APC Premium Grade

Pricing & Details

  
Prod No.
Size
Price
Avail.
Qty
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A107-0.25 mg
0.25 mg
$125.00
In stock
  

Antibody Details

Product Details

Host Species
Chicken Egg White
Product Concentration
1.0 mg/ml
Formulation
UltraAvidin-Allophycocyanin (APC) is supplied in 0.01M phosphate buffered saline (PBS) pH 7.4, containing 1.0% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This APC conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
See directions on lot specific datasheets, as information may periodically change.
Working Concentration
UltraAvidin-APC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. A working dilution ≤ 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications. However, each investigator should determine their own optimal working dilution for each specific research application.
Isoelectric Point
pI= 6.3 +/- 0.3
Specific Activity
11-17 µg biotin bound/ng UltraAvidin™
Each investigator should determine their own optimal working dilution for specific applications.
Excitation Laser
Red Laser (633 nm)

Description

Background
UltraAvidin™ is Leinco Technologies' trademark for a uniquely modified form of avidin which is isolated from chicken egg whites. With a molecular weight of 60,000, there are four identical subunits each capable of binding one molecule of biotin. UltraAvidin from Leinco Technologies has been de-glycosylated to prevent carbohydrate moieties from adhering to lectin-like receptors on the surface of cells, thus eliminating the possibility of false positives. Unlike native avidin, UltraAvidin has a near neutral pI which prevents electrostatic interactions with negatively charged serum or membrane proteins.

Recent studies have identified a universal recognition sequence in streptavidin which is similar to that found in several adhesion receptors.(1) It is a tripeptide sequence Arg-Tyr-Asp (RYD) which mimics the Arg-Gly-Asp (RGD) binding sequence of fibronectin. Strong interactions have been shown with streptavidin and cell surface molecules. This universal recognition sequence is not present in UltraAvidin. UltraAvidin may not significantly increase sensitivity in all research systems but will help assure specificity.

Antigen Details

References & Citations

1. Wilchek, M. et al. (1990) Biochem Biophys Res Commun. 170(3):1236-41.
2. Sullivan, W. et al. (1996) Genetics. 143(4):1629-42.
  
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.