Streptavidin – FITC - Leinco Technologies

Streptavidin – FITC

Product No.: S205


Product Type Avidin - Biotin


Select Product Size 1.0 mg — $110.00	Qty Max: Min: 1 Step: 1 $110.00 ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities

Data


Background Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (K_d~10^-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin. Protein Details Format Fluorescein (FITC) Product Concentration 0.2 mg/ml Formulation This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Working Concentration Streptavidin-FITC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. A woring dilution ≤ 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications. Storage and Stability This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day Ambient Certificate of Analysis Request COA IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Background

Streptavidin is a biotin binding protein present in the fermentation broth of the bacterium Streptomyces avidinii. Each molecule of streptavidin can bind four molecules of biotin with a high affinity constant (K_d~10^-15). Unlike native avidin, streptavidin is not glycosylated and has a near neutral isoelectric point (pI ~ 5-6) vs a pI of 10 for native avidin.

Protein Details

Format

Fluorescein (FITC)

Product Concentration

0.2 mg/ml

Formulation

This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.

Working Concentration

Streptavidin-FITC has been tested by flow cytometric analysis to detect biotinylated primary antibodies. A woring dilution ≤ 0.125 μg per 100 μl blood (or per 1 million cells in 100 μl total staining volume) is suitable for most applications.

Storage and Stability

This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.

Country of Origin

USA

Shipping

Next Day Ambient

Certificate of Analysis

Request COA

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.

Prod No.	Description
M1036	Goat Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Purified
M1049	Goat F(ab’)₂ Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Purified
R344	Goat F(ab’)₂ Anti-Rat IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Purified
H568	Goat F(ab’)₂ Anti-Human IgG, Fcγ Fragment Specific (Adsorbed Against: Ms., Bv., Hs.) – Purified
M356	Goat Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – FITC
H665	Goat F(ab’)₂ Anti-Human IgG, Fcγ Fragment Specific (Adsorbed Against: Ms., Bv., Hs.) – Biotin
H617	Goat F(ab’)2 Anti-Human IgG, Fcγ Fragment Specific (Adsorbed Against: Ms., Bv., Hs.) – HRP
M389	Goat Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – AMCA
R433	Goat F(ab’)₂ Anti-Rat IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Biotin
R389	Goat F(ab’)2 Anti-Rat IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – HRP
M1356	Goat F(ab’)₂ Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – DyLight^® 488
M1358	Goat F(ab’)₂ Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – DyLight^® 594
M1359	Goat F(ab')₂ Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – DyLight^® 650
M310	Goat Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Biotin
M1084	Goat Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – HRP
M287	Goat Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Alk Phos
R477	Goat F(ab’)2 Anti-Rat IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – FITC
M300	Goat F(ab’)₂ Anti-Mouse IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Alk Phos
R411	Goat F(ab’)₂ Anti-Rat IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – Alk Phos
H744	Goat F(ab’)₂ Anti-Human IgG, Fcγ Fragment Specific (Adsorbed Against: Ms., Bv., Hs.) – AMCA

Products are for research use only. Not for use in diagnostic or therapeutic procedures.

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Streptavidin – FITC