Goat F(ab’)2 Anti-Rat IgG, Fcγ Fragment Specific (Adsorbed Against: Hu., Bv., Hs.) – HRP

Purified Rat IgG, Fc (Gamma) Fragment (>98%)

This secondary antibody is lyophilized from 0.01M phosphate buffered saline (PBS) pH 7.6 containing 0.25M NaCl, 15 mg/ml IgG free BSA. (Warning: Use of sodium azide as a preservative will inhibit the enzyme activity of horseradish peroxidase)

This F(ab')₂ secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.

This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. Caution: This horseradish peroxidase conjugate does not contain preservatives. To avoid microbial contamination, store the reconstituted conjugate containing glycerol at -20°C in a manual defrost freezer.

One year from date of reconstitution

Next Day Ambient

It is recommended that working dilutions be prepared fresh daily.

WB: 1:5,000 - 1:100,000
WB Chemiluminescent: 1:10,000 - 1:200,000
ELISA: 1:5,000 - 1:100,000
IHC: 1:500 - 1:5,000

Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.

Goat F(ab')₂ Anti-Rat IgG, Fc (Gamma) Fragment recognizes Rat IgG, Fc (Gamma) Fragment. This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.