Anti-Mouse IL-4 (Clone 30340)
Anti-Mouse IL-4 (Clone 30340)
Product No.: I-662
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Clone 30340 Target IL-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names BCGF, BCDF, B Cell Stimulating Factor, BSF-1, Interleukin-4 Isotype IgG1 Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse IL-4 (>98%) Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830784 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is PN:I-252 at a concentration of approximately 0.1-0.4 µg/ml. A suggested standard for this assay is PN:I-207. Western Blotting: To detect Mouse IL-4 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-4 is 50 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-207. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse IL-4 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse IL-4 (at 7.5 ng/ml) is 0.07 - 0.15 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Clone 30340 recognizes an epitope on mouse IL-4. When used in combination with the biotinylated anti-mIL-4 detection antibody in sandwich ELISAs, no significant cross-reactivity or interference was observed with other cytokines tested. Background IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients. Antigen DetailsPubMed Research Area Other Molecules References & Citations1. Pike, JW. et al. (2001) PNAS 98: 2443 2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835 3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
