Anti-Mouse IL-4

Product No.: I-164

[product_table name="All Top" skus="I-164"]

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Target
IL-4
Product Type
Polyclonal Antibody
Alternate Names
Interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1
Applications
N
,
WB

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Goat
Immunogen
Purified Recombinant Mouse IL-4 (>98%)
Endotoxin Level
<0.01 EU/µg as determined by the LAL method
Formulation
This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.
State of Matter
Lyophilized
Storage and Handling
The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.
Country of Origin
USA
Shipping
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
Western Blot: Suitable for use at concentration of 0.1 µg/mL.
Additional Applications Reported In Literature ?
Neutralization: Measured by its ability to neutralize IL‑4-induced proliferation in the HT‑2 mouse T cell line. The Neutralization Dose (ND50) is typically 0.1-0.3 µg/mL in the presence of 7.5 ng/mL Recombinant Mouse IL‑4.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Mouse Interleukin-4 (IL-4) recognizes Mouse IL-4. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.
Background
IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients.

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Other Molecules

References & Citations

1. Pike, JW. et al. (2001) PNAS 98: 2443
2. Finkelman, FD. et al. (2003) J Immunol. 170: 3835
3. Kaplan, MH. et al. (2008) J Immunol. 180: 3551
N
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.