Recombinant Human IL-4

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Pricing & Details

Product. No.I-186
Alternate Names
interleukin-4, BCGF, BCDF, B Cell Stimulating Factor, BSF-1, MGC79402
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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I-186-10 µg
10 µg
$219.00
In stock
Max:
Min: 1
Step: 1
I-186-20 μg
20 µg
$289.00
In stock
Max:
Min: 1
Step: 1
I-186-50 µg
50 µg
$699.00
In stock
Max:
Min: 1
Step: 1

Background

IL-4 is a 15-19 kD pleiotropic immune cytokine secreted by activated TH2 cells. It has many biological roles, including the regulation in humoral and adaptive immunity, the inhibition of bone resorption, the stimulation of activated B-cell and T-cell proliferation, and initiation of allergic responses. Moreover, IL-4 induces a positive feedback loop by stimulating differentiation of naive helper T cells, or Th0 cells, to Th2 cells which in turn secrete more IL-4. Increased IL-4 production and overproduction of IL-4R are found in many cancers. IL-4 has a significant effect on tumor progression by increasing apoptosis resistance via tumor growth enhancement. IL-4 may also play a part in the immune dysregulation that occurs in most HIV-1 infected patients.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human IL-4 was determined by a cell proliferation assay using a human factor-dependent cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323). The expected ED50 for this effect is typically 0.05 - 0.2 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
mhkcdit lqeiiktlns lteqktlcte ltvtdifaas kntteketfc raatvlrqfy shhekdtrcl gataqqfhrh kqlirflkrl drnlwglagl nscpvkeanq stlenflerl ktimrekysk css
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human IL-4 is Mr 15 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 14 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives present.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Ødum, N. et al. (2001) Blood. 97(4):1056-62. Article Link
2. Ødum, N. et al. (2003) J Biol Chem. 278(5):2787-91. Article Link
3. Ødum, N et al. (2001) Leukemia 15:787–793 Article Link
4. Woetmann, A. et al. (2010) Experimental Dermatology 19:1096–1102. Article Link
5. Pike, JW. et al. (2001) PNAS 98:2443
6. Finkelman, FD. et al. (2003) J. Immunol. 170:3835
7. Paludan, SR. et al. (1998) Scand. J. Immunol. 48:459
8. Ryan, JJ. et al. (2007) Crit. Rev. Immunol. 27:15
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.