Anti-Akt (Phospho-Ser473) Antibody (43054)
Data
Immunohistochemistry: Detection of Akt (phosphoSer473) in paraffin-embedded
human lung carcinoma tissue.
Immunohistochemistry: Detection of Akt (phosphoSer473) in paraffin-embedded
human breast carcinoma tissue.
Immunoblotting: Detection of Akt (phosphoSer473) in extracts of 293 cells
untreated or treated with EGF or
calf intestinal phosphatase. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation sites of Serine 473 (Q-F-S(p)-Y-S) derived from human Akt and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A band of ~60kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100. These are recommended working dilutions. End user should determine optimal dilutions for their applications. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Akt (Phospho-Ser473) Background Akt, also known as Akt1 and Protein kinase B (PKB), is a serine/threonine-specific protein kinase that plays a key role in multiple cellular processes such as glucose metabolism, apoptosis, cell proliferation, transcription and cell migration. Akt1 is also able to induce protein synthesis pathways and is, therefore, a key signaling protein in the cellular pathways that lead to skeletal muscle hypertrophy, and general tissue growth. Since it can block apoptosis, and thereby promote cell survival, Akt1 has been implicated as a major factor in many types of cancer. Antigen DetailsUniProt.org Research Area Phosphospecific Antibodies References & CitationsTechnical Protocols  |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
