Anti-Bcl-xL (Phospho-Ser62) Antibody (43066)
Data
Immunofluorescence: Detection of Bcl-xL (phosphoSer62) in methanol-fixed HeLa
cells.
Immunohistochemistry: Detection of Bcl-xL (phosphoSer62) in paraffin-embedded
human breast carcinoma tissue.
Immunoblotting: Detection of Bcl-xL (phosphoSer62) in extracts of HeLa cells
untreated or treated with EGF. - -
- -
Antibody DetailsProduct DetailsReactivity Species Human ⋅ Mouse ⋅ Rat Host Species Rabbit Immunogen Peptide sequence that includes phosphorylation site of Serine 62 derived from human Bcl-xL and conjugated to KLH. Product Concentration 1.0 mg/ml Formulation PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. State of Matter Liquid Product Preparation Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide Storage and Handling This antibody is stable for at least one (1) year at -20°C. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at dilution of 1:500-1:1,000. A ban of ~30kDa is detected.
Immunohistochemistry: use at dilution of 1:50-1:100. Immunofluorescence: use at dilution of 1:100-1:200. These are recommended working dilutions. End user should determine optimal dilutions for their application. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Bcl-xL (Phospho-Ser62) Background B-cell lymphoma-extra large (Bcl-xl, or BCL2-like 1 isoform 1) is a transmembrane protein in the mitochondria. It is a member of the Bcl-2 family of proteins and acts as a pro-survival protein by preventing the release of mitochondrial cytochrome c, which would lead to caspase activation. Relative amounts of pro- and anti-survival Bcl-2 family proteins define whether a cell will undergo cell death: if more Bcl-xL is present, then pores are non-permeable to pro-apoptotic molecules and the cell survives. Antigen DetailsResearch Area Phosphospecific Antibodies References & CitationsWB,IHC,IF |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
