Anti-Ceramide Kinase Antibody (13805)

Product No.: 13805

- -
- -
Target
Ceramide Kinase
Product Type
Polyclonal
Alternate Names
hCERK, EC 2.7.1.138, Acylsphingosine kinase, Lipid kinase 4, LK4
Applications
IHC
,
IHC-P

- -
- -
Select Product Size

Data

Immunohistochemistry: Brain cortex: Formalin-fixed, paraffin-embedded
- -
- -

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Synthetic peptide conjugated to KLH.
Product Concentration
Lot Specific
Formulation
PBS containing 0.1% sodium azide
State of Matter
Liquid
Product Preparation
Purified by peptide immuno-affinity chromatography
Storage and Handling
This antibody is stable at 4°C (short- term) and -70°C (long-term).
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Immunohistochemistry: Use at 20ug/ml.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Ceramide Kinase
Background
Ceramide kinase (CERK), which phosphorylates ceramide to produce ceramide 1-phosphate (C1P), was first described as a Ca+2-stimulated lipid kinase that copurified with brain synaptic vesicles. CERK activity has been reported in HL60 cells, mast cells, and neutrophils. CERK is involved in phagolysosome formation in plymorphonuclear leukocytes and also in liposome fusion. C1P has been reported to have mitogenic effects. More recently, C1P was found to induce arachidonic acid release and prostanoid synthesis.

Antigen Details

Function
Catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate (PubMed:11956206, PubMed:16269826, PubMed:19168031). Acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8-dihydroceramide), to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines (PubMed:11956206, PubMed:16269826, PubMed:19168031). Shows a greater preference for D-erythro isomer of ceramides (PubMed:16269826). Binds phosphoinositides (PubMed:19168031). {PubMed:11956206, PubMed:16269826, PubMed:19168031}.
NCBI Gene Bank ID
UniProt.org
Research Area
Enzymes

References & Citations

IHC
IHC-P
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.