Anti-Human CD195 (CCR5) [Clone HEK/1/85a/7a] – Purified in vivo PLATINUM™ Functional Grade
Anti-Human CD195 (CCR5) [Clone HEK/1/85a/7a] – Purified in vivo PLATINUM™ Functional Grade
Product No.: I-2500
Clone HEK/1/85a/7a Target CD195 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names C-C chemokine receptor type 5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor Isotype Rat IgG2b κ Applications FC , ICC |
Antibody DetailsProduct DetailsReactive Species Human Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen CHO cells transfected with full-length human CD195 (CCR5). Product Concentration ≥ 5.0 mg/ml Endotoxin Level ≤ 0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2893834 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ? ICC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone HEK/1/85a/7a recognizes an epitope on human CCR5.
Background CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines1,2. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration3. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection8. CCR5 also contributes to other human diseases, including type I diabetes9 and cancer10.
Antigen Distribution CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs).
NCBI Gene Bank ID Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The clone HEK/1/85a/7a is specifically designed for in vivo mouse studies as a functional grade antibody targeting human CD195 (CCR5). Common applications of this antibody include:
Overall, this antibody is versatile and can be applied in various in vivo experiments to understand the role of CCR5 in biological processes and disease models. The most commonly used antibodies or proteins with HEK/1/85a/7a in the literature are those targeting various domains of the CCR5 receptor, with additional frequently employed reagents being anti-CD4 antibodies, anti-G protein antibodies, natural chemokine ligands, Env proteins/antibodies, and isotype controls. Key reagents and targets used alongside HEK/1/85a/7a:
Additional commonly encountered markers and proteins in related HEK cell line research:
Summary Table: Frequently Used Antibodies/Proteins with HEK/1/85a/7a
These reagents support immunophenotyping, receptor function analyses, and studies on viral infection mechanisms, most notably HIV-1 interactions via CCR5—with HEK/1/85a/7a serving as a pivotal monoclonal antibody for CCR5 detection and characterization. Citations involving clone HEK/1/85a/7a focus primarily on its role as a monoclonal antibody against human CCR5 (CD195), with broad applications in immunological and virological research, especially in the context of HIV, immune cell profiling, and disease characterization. Key findings from these citations include:
Additional technical details:
Summary of impact: Based on the available search results, there is no information available regarding dosing regimens of clone HEK/1/85a/7a across different mouse models. Clone HEK/1/85a/7a is identified as an anti-human CD195 (CCR5) monoclonal antibody with rat IgG2b κ isotype. While the search results provide detailed information about this antibody's characteristics, applications, and recommended concentrations for in vitro use (such as flow cytometry at ≤1.0 μg per 10⁶ cells), they explicitly state that no articles directly mention this clone, its dosing, or comparisons across different mouse models. The antibody recognizes an epitope on human CCR5, a chemokine receptor expressed on memory T cells, macrophages, and immature dendritic cells. However, the product information indicates that each investigator should determine their own optimal working dilution for specific applications, and titration is recommended for optimal performance. To establish appropriate dosing regimens for this antibody in mouse models, empirical studies would need to be conducted, taking into account factors such as the specific mouse strain, disease model, route of administration, and desired therapeutic or experimental endpoints. References & Citations1. Mackay CR., et al. (1997) Proc Natl Acad Sci. 94(5):1925-30 2. Doms RW., et al. (1999) Proc Natl Acad Sci. 96(9):5215-5220 3. Parmentier M., et al. (1996) Biochemistry. 35(11):3362-7 4. Berger EA., et al. (1996) Science. 272(5270):1955-8 5. Sodroski J., et al. (1996) Cell. 1996 85(7):1135-48 6. Doms RW., et al. (1996) Cell. 85(7):1149-58 7. Paxton WA., et al. (1996) Nature. 381(6584):667-73 8. Sheppard HW., et al. (1997) Nat Med. 3(10):1160-2 9. Delovitch TL., et al. (2000) J Immunol. 165(2):1102-10 10. Casagrande N., et al. (2020) Cancers (Basel). 12(7):1765 Technical Protocols ICC Certificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
