Anti-Human CD195 (CCR5) – Purified

Anti-Human CD195 (CCR5) – Purified

Product No.: I-2502

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Clone
HEK/1/85a/7a
Target
CD195
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
C-C chemokine receptor type 5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor
Isotype
IgG2b κ
Applications
FC
,
ICC

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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rat
Immunogen
CHO cells transfected with full-length human CD195 (CCR5).
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this CD195 antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Additional Applications Reported In Literature ?
ICC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone HEK/1/85a/7a recognizes an epitope on human CCR5.
Background
CCR5 antibody, HEK/1/85a/7a, recognizes C-C chemokine receptor type 5 (CCR5), also known as CD195, CC-CKR-5, CHEMR13, and HIV-1 fusion coreceptor. CCR5 is a 45 kDa seven-transmembrane G-protein-coupled receptor (GPCR). CCR5 is expressed on a subset of memory T cells, macrophages, and immature dendritic cells (DCs) and is upregulated by proinflammatory cytokines1,2. CCR5 binds to inflammatory CC-chemokines, including CCL3 (macrophage inflammatory protein (MIP)-1α), CCL4 (MIP-1β), and RANTES, which increases intracellular calcium levels and results in cytoskeletal rearrangement and chemotactic cell migration3. CCR5 is also the coreceptor for HIV-1 following binding of viral gp120 to CD44-7. The levels of CCR5 on cells influence the rate of entry of HIV-1, and patients with lower levels of CCR5 exhibit immunity against infection8. CCR5 also contributes to other human diseases, including type I diabetes9 and cancer10.
Antigen Distribution
CCR5 is expressed on memory T cells, macrophages, and immature dendritic cells (DCs).

Antigen Details

NCBI Gene Bank ID

References & Citations

1. Mackay CR., et al. (1997) Proc Natl Acad Sci. 94(5):1925-30
2. Doms RW., et al. (1999) Proc Natl Acad Sci. 96(9):5215-5220
3. Parmentier M., et al. (1996) Biochemistry. 35(11):3362-7
4. Berger EA., et al. (1996) Science. 272(5270):1955-8
5. Sodroski J., et al. (1996) Cell. 1996 85(7):1135-48
6. Doms RW., et al. (1996) Cell. 85(7):1149-58
7. Paxton WA., et al. (1996) Nature. 381(6584):667-73
8. Sheppard HW., et al. (1997) Nat Med. 3(10):1160-2
9. Delovitch TL., et al. (2000) J Immunol. 165(2):1102-10
10. Casagrande N., et al. (2020) Cancers (Basel). 12(7):1765
Flow Cytometry
ICC

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.