Anti-Human IgG2 [Clone HP6014] – Purified No Carrier Protein

Purified human immunoglobulin

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Liquid

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

Clone HP6014 activity is directed against the Fd portion of human IgG2, in the Fab region.

Humans produce five classes of immunoglobulins, IgM, IgD, IgA, IgE, and IgG¹. IgG is the most abundant class¹ and functions in the humoral immune response². There are four subclasses of IgG, and they are numbered according to decreasing abundance as IgG1 through IgG4. The four subclasses are highly conserved and consist of four polypeptide chains: two identical heavy chains and two identical light chains¹. While the general structure is very similar between IgG subclasses, differences in their constant regions in the hinge and upper CH2 domain as well as in glycosylation affect their binding to antigens (e.g. pathogenic proteins, polysaccharides, allergens), C1q and Fc receptors, leading to differences in functionality. As a result, each IgG subclass has a unique response skewed to specific antigens.

IgG2 responds to bacterial capsular polysaccharide antigens¹. Relative to the other IgG subclasses, IgG2 has a short, rigid hinge. Several structural hinge isomers exist, differing in the hinge region’s inter-chain disulfide bonds. IgG2 is less efficient at triggering C1q complement activation than IgG1 and IgG3 due to a leucine-to-alanine substitution at residue 235. Additionally, the short hinge may shield the C1q binding site. However, IgG2 can efficiently activate the complement cascade in the presence of high densities of polysaccharides. IgG2 also has a relatively reduced affinity for a number of FcγR.

HP6014 was generated by immunizing a BALB/c mouse with purified human immunoglobulin³. Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

IgG2 is produced by B cells and is present in serum.

IgG-Fc receptors, C1q

1 Vidarsson G, Dekkers G, Rispens T. Front Immunol. 5:520. 2014.
2 Rispens T, Huijbers MG. Nat Rev Immunol. 23(11):763-778. 2023.
3 Reimer CB, Phillips DJ, Aloisio CH, et al. Hybridoma. 3(3):263-275. 1984.
4 Jefferis R, Reimer CB, Skvaril F, et al. Immunol Lett. 10(3-4):223-252. 1985.