Anti-Human IL-2Rα (CD25) (Basiliximab) [Clone Hu107] — Dylight® 488
Anti-Human IL-2Rα (CD25) (Basiliximab) [Clone Hu107] — Dylight® 488
Product No.: LT311
Product No.LT311 Clone Hu107 Target CD25 Product Type Biosimilar Recombinant Human Monoclonal Antibody Alternate Names IL-2-RA; IL2-RA; TAC antigen; p55; CD25 Isotype Human IgG1κ Applications FC , IF , IHC |
Antibody DetailsProduct DetailsReactive Species Cynomolgus Monkey ⋅ Rhesus Monkey ⋅ Human Host Species Human Expression Host HEK-293 Cells FC Effector Activity Active Immunogen Human CD25 Product Concentration 0.2 mg/ml Formulation This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only (RUO). Non-Therapeutic. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (493 nm) RRIDAB_2893952 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for Basiliximab biosimilar antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IF IHC Additional Reported Applications For Relevant Conjugates ? CyTOF® WB B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Basiliximab. Basiliximab recognizes human CD25. This product is for research use only. Background CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2Rα. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25. Anti-Human IL-2R alpha (CD25) (Basiliximab) utilizes the same variable regions from the therapeutic antibody Basiliximab making it ideal for research projects. Antigen Distribution IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes. PubMed NCBI Gene Bank ID UniProt.org Research Area Biosimilars Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Research-grade Basiliximab biosimilars are used as analytical standards or controls in pharmacokinetic (PK) bridging ELISAs by serving as the reference material for constructing standard curves, calibrating assay responses, and enabling quantitative measurement of Basiliximab concentrations in serum samples. Context and Supporting Details:
Key Steps for Use in PK Bridging ELISA:
Summary Table: Basiliximab Biosimilar Use in PK ELISA
In summary, research-grade Basiliximab biosimilars serve as accessible, validated calibration and reference controls in PK bridging ELISAs, ensuring consistent, reproducible, and regulatory-aligned quantification of Basiliximab drug levels in serum samples. Standard flow cytometry protocols for validating CD25 (IL-2R alpha) expression or Basiliximab biosimilar binding capacity commonly use the conjugated antibody (e.g., PE or APC-labeled Basiliximab biosimilar) to stain cells expressing CD25, followed by quantitative analysis of cell surface expression levels. Typical Protocol Steps:
Validation/Experimental Controls:
Alternative Protocols for Non-Direct Conjugates:
Sample Protocol for Directly Conjugated Basiliximab Biosimilar:
Key Parameters and Notes:
References:
Analytical Assays for BiosimilarityBiopharmaceutical companies typically use a comprehensive set of analytical assays to confirm the structural and functional similarity of a proposed biosimilar to the originator drug. These assays include:
Leinco Biosimilar in StudiesThere is no specific information available about the "Leinco biosimilar" being used in these studies within the provided search results. However, the general principles of biosimilar development and characterization may still apply. Typically, biosimilars are compared to their reference products using the analytical methods mentioned above, regardless of the specific biosimilar manufacturer or product name. If "Leinco" refers to a specific manufacturer or product line, it would follow these standard analytical approaches to establish biosimilarity with an originator drug. However, without specific details, it's difficult to provide further insight into how a "Leinco biosimilar" is used in these studies. References & CitationsTechnical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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LT300 | |
LT303 | |
LT304 | |
LT311 | |
LT305 |
