Anti-Human IL-2Rα (CD25) (Basiliximab) [Clone Hu107] — PE
Anti-Human IL-2Rα (CD25) (Basiliximab) [Clone Hu107] — PE
Product No.: LT304
Product No.LT304 Clone Hu107 Target CD25 Product Type Biosimilar Recombinant Human Monoclonal Antibody Alternate Names IL-2-RA; IL2-RA; TAC antigen; p55; CD25 Isotype Human IgG1κ Applications FC , IF , IHC |
Antibody DetailsProduct DetailsReactive Species Cynomolgus Monkey ⋅ Rhesus Monkey ⋅ Human Host Species Human Expression Host HEK-293 Cells FC Effector Activity Active Immunogen Human CD25 Product Concentration 0.2 mg/ml Formulation This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze. Regulatory Status Research Use Only (RUO). Non-Therapeutic. Country of Origin USA Shipping Next Day 2-8°C Excitation Laser Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm) RRIDAB_2893950 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for Basiliximab biosimilar antibody for staining cells in flow cytometry is ≤ 1.0 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application. Additional Applications Reported In Literature ? IF IHC Additional Reported Applications For Relevant Conjugates ? CyTOF® WB B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This non-therapeutic biosimilar antibody uses the same variable region sequence as the therapeutic antibody Basiliximab. Basiliximab recognizes human CD25. This product is for research use only. Background CD25, a 55 kD type I transmembrane glycoprotein, has been shown to play roles in lymphocyte differentiation, activation, and proliferation. Many resting memory T cells constitutively express IL2Rα. It functions as the receptor for HTLV-1, resulting in its expression on neoplastic cells in adult T cell lymphoma/leukemia. CD25 (sIL-2R) has been used to track disease progression. Some additional clinical applications include Chagas disease, a disease characterized by a decline of CD25 expression on immune cells, and Multiple sclerosis, in which treatments with mAbs target CD25. Anti-Human IL-2R alpha (CD25) (Basiliximab) utilizes the same variable regions from the therapeutic antibody Basiliximab making it ideal for research projects. Antigen Distribution IL-2Rα is expressed on activated mature T and B lymphocytes, during early stages of thymocytes development, pre-B cells, and in activated CD4+ memory T-lymphocytes. PubMed NCBI Gene Bank ID UniProt.org Research Area Biosimilars Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Research-grade Basiliximab biosimilars are commonly used as calibration standards or reference controls in pharmacokinetic (PK) bridging ELISAs to accurately quantify Basiliximab concentrations in serum samples. In the context of a PK bridging ELISA:
PK Bridging ELISA workflow with biosimilar calibration standards:
Advantages of using research-grade biosimilars:
Additional context:
In summary, research-grade Basiliximab biosimilars act as reliable calibration standards and reference controls in PK bridging ELISAs, enabling accurate measurement of drug levels in serum samples for preclinical and clinical research applications. Standard flow cytometry protocols for using a conjugated Basiliximab biosimilar (such as PE or APC-labeled) to validate CD25 expression or assess binding capacity typically involve staining of target cells (e.g., PBMCs or transfected cell lines) and quantifying specific antibody binding via fluorescence intensity. Protocol Overview:
Critical Details and Controls:
Applications:
Sample Protocol (based on manufacturer and literature):
To summarize: Biopharma companies employ a comprehensive battery of analytical assays to demonstrate that proposed biosimilars are highly similar to their reference products in both structure and function. These assessments form the foundation of biosimilar development and regulatory approval. Primary Structure CharacterizationThe analytical similarity assessment begins with confirming that the biosimilar has an identical primary amino acid sequence to the reference product. Peptide mapping using liquid chromatography-mass spectrometry (LC-MS) serves as the gold standard technique for this analysis. This method fragments the protein into smaller peptides and analyzes each piece to ensure complete sequence identity. Higher-Order Structure AnalysisBeyond primary structure, companies must demonstrate that the three-dimensional folding and conformation of the biosimilar matches the reference product. Key analytical techniques include:
Post-Translational Modifications AssessmentPost-translational modifications significantly impact protein function and immunogenicity. Critical assays include:
Purity and Impurity ProfilingThe analytical similarity assessment must include rigorous comparison of purity and impurity profiles between the biosimilar and reference product. Key techniques encompass:
Functional CharacterizationDemonstrating structural similarity is necessary but insufficient - the biosimilar must also exhibit equivalent biological function. Comprehensive functional testing includes: Binding Assays:
Potency and Activity Assays:
Statistical and Comparative AnalysisManufacturers conduct head-to-head comparisons of the proposed biosimilar's properties against the reference product, with results required to fall within appropriate limits, ranges, or distributions. This extensive characterization uses highly sensitive analytical methods to measure molecular properties across multiple lots of both products. The analytical studies focus on critical quality attributes (CQAs) that are ranked by their risk of impact on the product's activity, pharmacokinetics, pharmacodynamics, safety, efficacy, or immunogenicity. This risk-based approach ensures that the most important molecular properties receive particular attention during the similarity assessment. Regarding the Leinco biosimilar, the search results do not contain any specific information about this particular product or its use in analytical studies. The analytical techniques and approaches described above represent the standard industry practices that would typically be applied to any biosimilar development program, but without additional information, I cannot provide details about how Leinco specifically utilizes these methods in their biosimilar studies. References & CitationsTechnical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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LT300 | |
LT303 | |
LT304 | |
LT311 | |
LT305 |
