Anti-Ly49C (Clone 4LO3311) – Purified in vivo GOLD™ Functional Grade

In in vivo mouse studies, clone 4LO3311 is used as a functional grade monoclonal antibody to specifically target Ly49C, a receptor expressed on natural killer (NK) cells, uterine NK cells, NKT cells, and CD8+ T cells. The antibody is applied to modulate NK cell function by binding an epitope in the stalk region adjacent to the carbohydrate recognition domain of Ly49C.

• Key applications include:

  • Studying the role of Ly49C in regulating NK cell and T cell activity—for example, inhibiting NK cell cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) in specific mouse strains (C57BL/6 and C3H).
  • Identifying and depleting Ly49C-positive populations for functional analyses, including NK cell subset characterization, immune cell phenotyping, or mechanistic studies of innate immunity.
  • Blocking Ly49C-mediated inhibitory signaling in immune response and tumor immunology models.

• Characteristics for in vivo use:

  • Supplied as mouse IgG3 kappa in low or ultra-low endotoxin preparations for minimal immunogenic response and optimal safety in animal studies.
  • Pathogen-tested to ensure suitability for animal experiments.
  • Distributed in bulk sizes for experimental scalability.

• Experimental notes:

  • 4LO3311 was developed by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells, optimizing its specificity for Ly49C and minimizing cross-reactivity with other Ly49 family members.
  • Its binding profile allows selective inhibition or depletion of Ly49C-expressing cells without affecting other Ly49 isoforms, making it valuable for dissecting Ly49C-specific pathways.

In summary, clone 4LO3311 is used to probe Ly49C function in mouse models by enabling depletion, inhibition, or phenotyping of Ly49C+ immune cells, with robust specificity and in vivo suitability.

The correct storage temperature for sterile packaged clone 4LO3311 (such as anti-Ly49C, clone 4LO3311) is 2–8°C (refrigerated conditions); it should not be frozen.

• Store at 2–8°C under sterile conditions as supplied.
• Do not freeze.
• If stored at this temperature, stability and function are maintained for at least 1 month.
• For long-term storage (12 months), -70°C can be used, but this is typically for more specialized applications and should avoid freeze-thaw cycles.
• Always follow lot-specific datasheet instructions, as protocols may vary slightly by formulation or conjugate.

This storage condition is standard for fluorescently labeled monoclonal antibodies supplied sterile and ensures product integrity and performance.

Commonly used antibodies or proteins used alongside 4LO3311 (anti-Ly49C) in the literature, particularly in murine NK cell studies, typically include other antibodies targeting NK cell receptors, T cell markers, and related Ly49 family members. According to product descriptions and cited research:

• Other Ly49 family antibodies: Because Ly49C is closely related to Ly49F, Ly49I, and Ly49H, studies often include antibodies against these family members to distinguish receptor expression profiles or to study NK cell diversity.
• NK cell/T cell markers: Antibodies against NK1.1, CD3, CD8, and CD4 are commonly used to differentiate NK, NKT, or T cell populations when assessing Ly49C expression.
• MHC class I antibodies: Because Ly49 receptors, including Ly49C, interact with MHC class I molecules, studies may employ antibodies against H2-Kb or other MHC class I proteins to investigate binding and functional responses.
• Other functional antibodies: In functional assays, those investigating cytotoxicity or regulation use complement, anti-IFN?, anti-TNF?, or antibodies inflicting ADCC or complement-mediated lysis, often combining 4LO3311 with such reagents.

Summary table:

Scientific articles and suppliers corroborate that co-staining and combinatorial analysis with these antibodies are routine in studies leveraging clone 4LO3311. Most studies will also include isotype controls and viability dyes for flow cytometry, though these are generic reagents not specific to Ly49C research.

If you need specifics from particular experimental contexts (e.g., in vivo depletion, flow cytometry panels), please clarify for more targeted information.

Key findings from clone 4LO3311 citations in scientific literature highlight its utility as a research tool for identifying and functionally characterizing Ly49C, an inhibitory receptor on mouse NK cells. The main outcomes cited in scientific literature using 4LO3311 are:

• Specificity: Clone 4LO3311 is a monoclonal antibody directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1), binding a unique epitope in the stalk region near the carbohydrate recognition domain of the Ly49C molecule.
• Functional Activity: 4LO3311, especially when used with complement, can inhibit NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H mouse spleen cells. It selectively inhibits NK cell activity in C57BL/6 and C3H mice but shows no inhibitory effect on NZB NK cells.
• Epitope Recognition: The antibody recognizes a 32-amino acid segment adjacent to the carbohydrate recognition domain, allowing mapping of Ly49C expression without cross-reactivity to closely related Ly49 family members such as Ly49A, B, D, E, F, G, and H in certain genetic backgrounds.
• Cellular Distribution: Ly49C, as labeled by 4LO3311, is expressed on NK cells, uterine NK cells, NKT cells, and a subset of CD8+ Tregs.
• Role in Immunology: Ly49C functions as an inhibitory receptor important for NK cell education and tuning the activation threshold by interacting with class I MHC; this is critical for regulation of antiviral responses and immune homeostasis.
• Experimental References:
  • Lemieux et al. (1991) established the original characterization of 4LO3311, defining its specificity and inhibitory properties on NK cell cytotoxicity.
  • Brennan et al. (1996) further mapped the epitope recognized by 4LO3311, contributing to understanding of Ly49 receptor diversity.

These publications have established 4LO3311 as a key reagent for dissecting the function of Ly49C in mouse models, with ongoing relevance for studies of NK cell regulation, immune response to infection, and cancer immunology. Recent comprehensive reviews and primary research continue to cite work involving 4LO3311 when describing Ly49C function and NK cell biology.