Anti-Ly49C (Clone 4LO3311) – Purified in vivo GOLD™ Functional Grade

Anti-Ly49C (Clone 4LO3311) – Purified in vivo GOLD™ Functional Grade

Product No.: L244

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Clone
4LO3311
Target
Ly-49C
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Ly-49 inhibitory receptors C
Isotype
Mouse IgG3κ
Applications
Depletion
,
FC
,
in vivo
,
IP

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
4LO3311 activity is directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1). 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain.
Background
Ly49 receptors are homodimeric type II C-type lectin-like membrane glycoproteins encoded by a family of highly polymorphic genes in the mouse natural killer (NK) gene complex1. Ly49 receptors recognize class I major histocompatibility complex-I (MHC-1) and MHC-1 like proteins. Ly49C is an inhibitory member of the Ly-49 superfamily. Inhibitory Ly49 receptors are involved in NK cell education1 and play a role in controlling viral infection2. Additionally, Ly49C decreases the activation threshold of NK cells by inhibiting H2-Kb 3. Ly49C is expressed on NK, uterine NK, NKT, and CD8+ Tregs cells1. Ly49C is closely related to Ly49F, Ly49I, and Ly49H3. 4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells4. Hybridoma lines were prepared by fusing immune spleen cells with non-secreting P3X63-Ag8.653 myeloma cells and subsequently screened for production of anti-NK antibodies. Isotype was determined by double-immunodiffusion analysis of hybridoma supernatants using rabbit monospecific anti-mouse immunoglobulins. 4LO3311 is of IgG3, κ isotype. 4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain5. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H. 4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.
Antigen Distribution
Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells.
Research Area
Immunology

Leinco Antibody Advisor

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In in vivo mouse studies, clone 4LO3311 is used as a functional grade monoclonal antibody to specifically target Ly49C, a receptor expressed on natural killer (NK) cells, uterine NK cells, NKT cells, and CD8+ T cells. The antibody is applied to modulate NK cell function by binding an epitope in the stalk region adjacent to the carbohydrate recognition domain of Ly49C.

  • Key applications include:

    • Studying the role of Ly49C in regulating NK cell and T cell activity—for example, inhibiting NK cell cytotoxicity and antibody-dependent cellular cytotoxicity (ADCC) in specific mouse strains (C57BL/6 and C3H).
    • Identifying and depleting Ly49C-positive populations for functional analyses, including NK cell subset characterization, immune cell phenotyping, or mechanistic studies of innate immunity.
    • Blocking Ly49C-mediated inhibitory signaling in immune response and tumor immunology models.
  • Characteristics for in vivo use:

    • Supplied as mouse IgG3 kappa in low or ultra-low endotoxin preparations for minimal immunogenic response and optimal safety in animal studies.
    • Pathogen-tested to ensure suitability for animal experiments.
    • Distributed in bulk sizes for experimental scalability.
  • Experimental notes:

    • 4LO3311 was developed by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells, optimizing its specificity for Ly49C and minimizing cross-reactivity with other Ly49 family members.
    • Its binding profile allows selective inhibition or depletion of Ly49C-expressing cells without affecting other Ly49 isoforms, making it valuable for dissecting Ly49C-specific pathways.

In summary, clone 4LO3311 is used to probe Ly49C function in mouse models by enabling depletion, inhibition, or phenotyping of Ly49C+ immune cells, with robust specificity and in vivo suitability.

The correct storage temperature for sterile packaged clone 4LO3311 (such as anti-Ly49C, clone 4LO3311) is 2–8°C (refrigerated conditions); it should not be frozen.

  • Store at 2–8°C under sterile conditions as supplied.
  • Do not freeze.
  • If stored at this temperature, stability and function are maintained for at least 1 month.
  • For long-term storage (12 months), -70°C can be used, but this is typically for more specialized applications and should avoid freeze-thaw cycles.
  • Always follow lot-specific datasheet instructions, as protocols may vary slightly by formulation or conjugate.

This storage condition is standard for fluorescently labeled monoclonal antibodies supplied sterile and ensures product integrity and performance.

Commonly used antibodies or proteins used alongside 4LO3311 (anti-Ly49C) in the literature, particularly in murine NK cell studies, typically include other antibodies targeting NK cell receptors, T cell markers, and related Ly49 family members. According to product descriptions and cited research:

  • Other Ly49 family antibodies: Because Ly49C is closely related to Ly49F, Ly49I, and Ly49H, studies often include antibodies against these family members to distinguish receptor expression profiles or to study NK cell diversity.
  • NK cell/T cell markers: Antibodies against NK1.1, CD3, CD8, and CD4 are commonly used to differentiate NK, NKT, or T cell populations when assessing Ly49C expression.
  • MHC class I antibodies: Because Ly49 receptors, including Ly49C, interact with MHC class I molecules, studies may employ antibodies against H2-Kb or other MHC class I proteins to investigate binding and functional responses.
  • Other functional antibodies: In functional assays, those investigating cytotoxicity or regulation use complement, anti-IFN?, anti-TNF?, or antibodies inflicting ADCC or complement-mediated lysis, often combining 4LO3311 with such reagents.

Summary table:

Antibody/ProteinPurpose/Context
Ly49F, Ly49I, Ly49HDiscrimination of Ly49 family members
NK1.1, CD3, CD8, CD4NK/T cell population identification
H2-Kb (MHC I)Assessing Ly49–MHC interactions
Complement/ADCC AbsFunctional cytotoxicity assays
Anti-IFN?, anti-TNF?Cytokine response/functional readout

Scientific articles and suppliers corroborate that co-staining and combinatorial analysis with these antibodies are routine in studies leveraging clone 4LO3311. Most studies will also include isotype controls and viability dyes for flow cytometry, though these are generic reagents not specific to Ly49C research.

If you need specifics from particular experimental contexts (e.g., in vivo depletion, flow cytometry panels), please clarify for more targeted information.

Key findings from clone 4LO3311 citations in scientific literature highlight its utility as a research tool for identifying and functionally characterizing Ly49C, an inhibitory receptor on mouse NK cells. The main outcomes cited in scientific literature using 4LO3311 are:

  • Specificity: Clone 4LO3311 is a monoclonal antibody directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1), binding a unique epitope in the stalk region near the carbohydrate recognition domain of the Ly49C molecule.
  • Functional Activity: 4LO3311, especially when used with complement, can inhibit NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H mouse spleen cells. It selectively inhibits NK cell activity in C57BL/6 and C3H mice but shows no inhibitory effect on NZB NK cells.
  • Epitope Recognition: The antibody recognizes a 32-amino acid segment adjacent to the carbohydrate recognition domain, allowing mapping of Ly49C expression without cross-reactivity to closely related Ly49 family members such as Ly49A, B, D, E, F, G, and H in certain genetic backgrounds.
  • Cellular Distribution: Ly49C, as labeled by 4LO3311, is expressed on NK cells, uterine NK cells, NKT cells, and a subset of CD8+ Tregs.
  • Role in Immunology: Ly49C functions as an inhibitory receptor important for NK cell education and tuning the activation threshold by interacting with class I MHC; this is critical for regulation of antiviral responses and immune homeostasis.
  • Experimental References:
    • Lemieux et al. (1991) established the original characterization of 4LO3311, defining its specificity and inhibitory properties on NK cell cytotoxicity.
    • Brennan et al. (1996) further mapped the epitope recognized by 4LO3311, contributing to understanding of Ly49 receptor diversity.

These publications have established 4LO3311 as a key reagent for dissecting the function of Ly49C in mouse models, with ongoing relevance for studies of NK cell regulation, immune response to infection, and cancer immunology. Recent comprehensive reviews and primary research continue to cite work involving 4LO3311 when describing Ly49C function and NK cell biology.

References & Citations

1. Rahim MM, Tu MM, Mahmoud AB, et al. Front Immunol. 5:145. 2014.
2. Parikh BA, Bern MD, Piersma SJ, et al. Cell Rep. 32(4):107969. 2020.
3. Schenkel AR, Kingry LC, Slayden RA. Front Immunol. 4:90. 2013.
4. Lemieux S, Ouellet-Talbot F, Lusignan Y, et al. Cell Immunol. 134(1):191-204. 1991.
5. Brennan J, Lemieux S, Freeman JD, et al. J Exp Med. 184(6):2085-2090. 1996.
6. Hsu J, Hodgins JJ, Marathe M, et al. J Clin Invest. 128(10):4654-4668. 2018.
7. Depatie C, Chalifour A, Paré C, et al. Int Immunol. 11(9):1541-1551. 1999.
Depletion
Flow Cytometry
in vivo Protocol
Immunoprecipitation Protocol

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.