Anti-Ly49C (Clone 4LO3311) – Purified in vivo GOLD™ Functional Grade
Anti-Ly49C (Clone 4LO3311) – Purified in vivo GOLD™ Functional Grade
Product No.: L244
Clone 4LO3311 Target Ly-49C Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Ly-49 inhibitory receptors C Isotype Mouse IgG3κ Applications Depletion , FC , in vivo , IP |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 4LO3311 activity is directed against Ly49C (Killer cell lectin-like receptor 3, KLRA3; NK2.1). 4LO3311 binds an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain. Background Ly49 receptors are homodimeric type II C-type lectin-like membrane glycoproteins encoded by a family of highly polymorphic genes in the mouse natural killer (NK) gene complex1. Ly49 receptors recognize class I major histocompatibility complex-I (MHC-1) and MHC-1 like proteins. Ly49C is an inhibitory member of the Ly-49 superfamily. Inhibitory Ly49 receptors are involved in NK cell education1 and play a role in controlling viral infection2. Additionally, Ly49C decreases the activation threshold of NK cells by inhibiting H2-Kb 3. Ly49C is expressed on NK, uterine NK, NKT, and CD8+ Tregs cells1. Ly49C is closely related to Ly49F, Ly49I, and Ly49H3.
4LO3311 was generated by immunizing 129/SvJ mice with C57BL/6 NK-enriched spleen cells4. Hybridoma lines were prepared by fusing immune spleen cells with non-secreting P3X63-Ag8.653 myeloma cells and subsequently screened for production of anti-NK antibodies. Isotype was determined by double-immunodiffusion analysis of hybridoma supernatants using rabbit monospecific anti-mouse immunoglobulins. 4LO3311 is of IgG3, κ isotype. 4LO3311 recognizes an epitope located in a 32-amino acid segment of the stalk region immediately adjacent to the carbohydrate recognition domain5. 4LO3311 recognizes Ly-49CBALB but not Ly-49AB6, Ly-49ABALB, BBALB, DB6, EB6, FB6, GB6, GBALB, or H.
4LO3311 plus complement inhibits NK cell and antibody-dependent cellular cytotoxicity (ADCC) activity in C3H spleen cells4. 4LO3311 inhibits C57BL/6 and C3H NK cell activity but has no effect on NZB NK cells.
Antigen Distribution Ly49C is expressed on NK, uterine NK, NKT, and CD8+ T cells. Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. In in vivo mouse studies, clone 4LO3311 is used as a functional grade monoclonal antibody to specifically target Ly49C, a receptor expressed on natural killer (NK) cells, uterine NK cells, NKT cells, and CD8+ T cells. The antibody is applied to modulate NK cell function by binding an epitope in the stalk region adjacent to the carbohydrate recognition domain of Ly49C.
In summary, clone 4LO3311 is used to probe Ly49C function in mouse models by enabling depletion, inhibition, or phenotyping of Ly49C+ immune cells, with robust specificity and in vivo suitability. The correct storage temperature for sterile packaged clone 4LO3311 (such as anti-Ly49C, clone 4LO3311) is 2–8°C (refrigerated conditions); it should not be frozen.
This storage condition is standard for fluorescently labeled monoclonal antibodies supplied sterile and ensures product integrity and performance. Commonly used antibodies or proteins used alongside 4LO3311 (anti-Ly49C) in the literature, particularly in murine NK cell studies, typically include other antibodies targeting NK cell receptors, T cell markers, and related Ly49 family members. According to product descriptions and cited research:
Summary table:
Scientific articles and suppliers corroborate that co-staining and combinatorial analysis with these antibodies are routine in studies leveraging clone 4LO3311. Most studies will also include isotype controls and viability dyes for flow cytometry, though these are generic reagents not specific to Ly49C research. If you need specifics from particular experimental contexts (e.g., in vivo depletion, flow cytometry panels), please clarify for more targeted information. Key findings from clone 4LO3311 citations in scientific literature highlight its utility as a research tool for identifying and functionally characterizing Ly49C, an inhibitory receptor on mouse NK cells. The main outcomes cited in scientific literature using 4LO3311 are:
These publications have established 4LO3311 as a key reagent for dissecting the function of Ly49C in mouse models, with ongoing relevance for studies of NK cell regulation, immune response to infection, and cancer immunology. Recent comprehensive reviews and primary research continue to cite work involving 4LO3311 when describing Ly49C function and NK cell biology. References & Citations1. Rahim MM, Tu MM, Mahmoud AB, et al. Front Immunol. 5:145. 2014.
2. Parikh BA, Bern MD, Piersma SJ, et al. Cell Rep. 32(4):107969. 2020. 3. Schenkel AR, Kingry LC, Slayden RA. Front Immunol. 4:90. 2013. 4. Lemieux S, Ouellet-Talbot F, Lusignan Y, et al. Cell Immunol. 134(1):191-204. 1991. 5. Brennan J, Lemieux S, Freeman JD, et al. J Exp Med. 184(6):2085-2090. 1996. 6. Hsu J, Hodgins JJ, Marathe M, et al. J Clin Invest. 128(10):4654-4668. 2018. 7. Depatie C, Chalifour A, Paré C, et al. Int Immunol. 11(9):1541-1551. 1999. Technical ProtocolsCertificate of Analysis |
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