Anti-Mouse CD137L (4-1BBL) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD137L (4-1BBL) – Purified in vivo GOLD™ Functional Grade
Product No.: L322
Clone TKS-1 Target 4-1BBL Formats AvailableView All Product Type Monoclonal Antibody Alternate Names 4-1BBL, CD137L, TNFSF9, 4-1BB Ligand, TKS-1 Isotype Rat IgG2a κ Applications FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen BALB/c mouse B lymphoma line 2PK-3 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Additional Applications Reported In Literature ? FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity TKS-1 activity is directed against 4-1BBL (CD137L). Background 4-1BBL (CD137L) and 4-1BB (CD137) are a receptor ligand pair belonging to the tumor necrosis factor (TNF) receptor/TNF superfamily1,2. 4-1BBL is a type II transmembrane protein expressed by splenic B and T cells, macrophages, and dendritic cells1-5. Members of the TNF-TNF receptor superfamily play critical roles in regulating cellular activation, differentiation, and apoptosis6 and the 4-1BBL-4-1BB interaction is important in cellular immune responses5. 4-1BBL-4-1BB interaction provides a co-stimulatory signal to T cells, resulting in increased proliferation and cytokine production5.
A TKS-1-producing hybridoma was generated by immunizing a SD rat with 4-1BBL-transfected NRK cells (rat IgG2a,κ)5. Splenocytes were fused with P3U1 cells and hypoxanthine–aminopterin–thymidine selection was performed. The monoclonal antibody was identified by its strong reactivity with 4-1BBL-transfected L5178Y cells, cloned by limiting diffusion, purified from ascites, and found to bind to 4-1BBL/NRK and 4-1BBL/P815 cells5. Pre-incubation with TKS-1 blocks 4-1BB–Ig binding to 4-1BBL/L5178Y cells, indicating TKS-1 is specific to mouse 4-1BBL and can interrupt the interaction between 4-1BBL and 4-1BB5. Additionally, TKS-1 can block anti-CD3-stimulated T cell proliferation co-stimulated by 4-1BBL /P815 cells. The monoclonal antibodies 19H3 and TKS-1 bind to different sites on murine 4-1BBL, with TKS-1 binding to a site that overlaps with the receptor binding site7. Additionally, TKS-1 and 4-1BB bind to a similar site, making TKS-1 especially useful for blocking studies. Antigen Distribution 4-1BBL is expressed by activated B cells, macrophages, and dendritic cells. Ligand/Receptor 4-1BB (CDw137) Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The TKS-1 clone is used in in vivo mouse studies primarily for targeting the mouse 4-1BB ligand (4-1BBL), also known as CD137L. This ligand is crucial in immune responses, particularly in the activation and survival of T cells, including CD8+ T cells, which are involved in antitumor immunity. Role of 4-1BBL and TKS-1:
In Vivo Applications:
Overall, the TKS-1 clone is a valuable tool in in vivo mouse studies for understanding and manipulating immune responses, particularly in the context of cancer research and immunotherapy. The search results do not provide specific information about the storage temperature for a sterile packaged clone TKS-1. However, for general biological materials, especially DNA or bacteria, it is common to store them at -20°C to preserve their integrity and viability. For specific instructions on the storage of TKS-1, it would be best to consult the manufacturer's guidelines or the product documentation provided with the clone. If TKS-1 is related to biological vectors similar to the pCold System, it might require storage at -20°C, similar to the pCold DNA vectors. Commonly used antibodies or proteins alongside TKS-1 in the literature include co-stimulatory and checkpoint molecules, as well as T-cell markers and peptide antigens involved in immune response modulation. Key antibodies and proteins cited with TKS-1:
In summary, studies employing TKS-1 commonly use antibodies or proteins such as 3H3, CD8, CD62L, CCR7, S1PR1, Thy1.1, and relevant antigenic peptides (e.g., mgp100), often in mouse models of T-cell activation, differentiation, and immunotherapy. The key findings from scientific literature citing clone TKS-1 indicate that this antibody induces only mild agonistic signaling of 4-1BB compared to other clones, such as 3H3, which strongly triggers 4-1BB signals with minimal or no 4-1BBL signals. TKS-1 is used experimentally to study the function and signaling of 4-1BB (CD137) in immune cells, particularly in models assessing self-reactive CD8+ T cell survival and immune regulation.
These findings have contributed to immunological research, particularly in deciphering costimulatory signal modulation and the potential therapeutic manipulation of T cell responses. There is no evidence in indexed literature linking clone TKS-1 to replication controversies or citation impact debates relevant to nonreplicable publications. References & Citations1. Goodwin RG, Din WS, Davis-Smith T, et al. Eur. J. Immunol. 23:2631-2641. 1993.
2. Alderson MR, Smith CA, Tough TW, et al. Eur. J. Immunol. 24:2219-2227. 1994. 3. Pollok KE, Kim YJ, Hurtado J, et al. Eur J Immunol. 24(2):367-374. 1994. 4. DeBenedette MA, Shahinian A, Mak TW, et al. J. Immunol. 158:551-559. 1997. 5. Futagawa T, Akiba H, Kodama T, et al. Int Immunol. 14(3):275-286. 2002. 6. Smith CA, Farrah T, Goodwin RG. Cell 76:959-962. 1994. 7. Mbanwi AN, Lin GHY, Wang KC, et al. J Immunol Methods. 450:81-89. 2017. 8. Zheng G, Wang B, Chen A. J Immunol. 173(4):2428-2434. 2004. 9. Madireddi S, Eun SY, Lee SW, et al. J Exp Med. 211(7):1433-1448. 2014. 10. Shrestha S, Noh JM, Kim SY, et al. Oncoimmunology. 5(1):e1067744. 2015. 11. Kang SW, Lee SC, Park SH, et al. Cancer Res. 77(21):5989-6000. 2017. Technical ProtocolsCertificate of Analysis |
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