Anti-Mouse CD90 (Thy-1) (Clone M5/49.4.1) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD90 (Thy-1) (Clone M5/49.4.1) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C645
Clone M5/49.4.1 Target Thy1 (CD90) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Thy-1 membrane glycoprotein, CD90 Isotype Rat IgG2a Applications Depletion , FA , IP |
Antibody DetailsProduct DetailsHost Species Rat Recommended Dilution Buffer Immunogen Secondary mixed lymphocyte culture consisting of C57BL/6 mouse anti-irradiated BN rat lymphoma Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? Depletion, FA, IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity M5/49.4.1 activity is directed against mouse Thy1 (CD90). Background Thy1 is a highly conserved, GPI-linked member of the immunoglobulin superfamily that is
important in the immune and nervous systems1 and involved in T-cell activation and cell-cell
interactions2. The effects of Thy1 are context-dependent1. Thy1 is heavily N-glycosylated with a
carbohydrate content of up to 40% of its molecular mass, and its moiety composition varies
between tissues as well as between cells of the same lineage in different stages of differentiation.
Additionally, Thy1 is found in both membrane-bound and soluble forms, and, in mice, Thy1 is
encoded by two alleles, Thy1.1 and Thy1.2, which are distinguished by a single amino acid at
position 891. Thy1 deficiency does not compromise immunity2, but its presence or absence
modulates the phenotypes of certain cancers, fibrotic diseases, and neuronal injury1. Thy1.1 is an
alloantigen of the AKR/J and PL mouse strains, whereas Thy1.2 is expressed by most mouse
strains2,3. M5/49.4.1 was generated by immunizing (Lewis x BN) F1 rats with a secondary mixed lymphocyte culture consisting of C57BL/6 mouse anti-irradiated BN rat lymphoma4. Spleen cells were fused with NS-1 myeloma cells and hybridomas grown and assessed for binding to cytolytic T lymphocyte (CTL) surface molecules. Antigen Distribution CD90 is expressed in mice by thymocytes, peripheral T-cells, myoblasts,
epidermal cells, and keratinocytes. CD90 is considered a pan T cell marker of mice. Ligand/Receptor CD45 NCBI Gene Bank ID Research Area Immunology . Signal Transduction . Immunoglobulins Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Applications of M5/49.4.1 Clone in In Vivo Mouse StudiesAntibody Properties and Specificity The M5/49.4.1 monoclonal antibody specifically targets the mouse Thy-1 (CD90) antigen, a glycosylphosphatidylinositol (GPI)-linked glycoprotein expressed on various cell types, including T cells, thymocytes, myoblasts, and certain epithelial cells. This rat IgG2a, ? isotype antibody is validated for mouse reactivity and is available in multiple formats (e.g., FITC, APC, purified for functional in vivo use). In Vivo Functional Applications While most references emphasize its use in flow cytometry, immunohistochemistry, and cell depletion assays in vitro, the purified "in vivo GOLD Functional Grade" format specifically indicates suitability for in vivo mouse studies. These high-purity, low-endotoxin preparations are designed for administration directly into the mouse, where they can mediate cell depletion or block CD90 function in experimental models. Typical In Vivo Uses
Key Considerations
Summary Table: M5/49.4.1 In Vivo Applications
ConclusionThe M5/49.4.1 clone is a versatile tool for in vivo mouse studies, primarily used for depleting or blocking CD90-positive cells to investigate immune, fibrotic, or neurodegenerative processes. Its high specificity for mouse Thy-1 and availability in formats suitable for direct in vivo use make it a valuable reagent for mechanistic and translational research. Correct Storage Temperature for Clone M5/49.4.1 (Sterile Packaged Antibody)For the sterile packaged monoclonal antibody clone M5/49.4.1, which recognizes mouse CD90 (Thy1), the manufacturers instructions specify the following storage conditions:
The optimal storage temperature is thus 2–8°C, which is standard for most monoclonal antibody solutions shipped in a sterile, liquid form. If the product is supplied as a pellet, powder, or lyophilized, always refer to the manufacturers data sheethowever, based on the information available, this clone is typically distributed in liquid solution. Summary Table
For long-term storage and maximal stability, avoid repeated freeze-thaw cycles and keep the vial tightly closed when not in use. Note: If the product is not specifically packaged as a sterile liquid or is in a different form, always check the product datasheet for the most accurate, up-to-date storage instructions. The information above is based on currently available commercial sources for this clone. Based on the available information, M5/49.4.1 (anti-mouse CD90/Thy1 antibody) is commonly used alongside several other antibodies and proteins in immunological research, particularly for cell depletion and characterization studies. Complement ComponentsM5/49.4.1 is frequently used in conjunction with complement for T cell depletion protocols. This combination enhances the cytotoxic effect of the antibody, as complement-dependent cytotoxicity (CDC) is one of the primary mechanisms through which depletion antibodies function. Other Cell Depletion AntibodiesIn comprehensive immune cell depletion studies, M5/49.4.1 is often used alongside other T cell-targeting antibodies, including:
Bone Marrow Transfer ExperimentsIn bone marrow chimera studies, M5/49.4.1 is used to treat donor bone marrow cells before transfer. In these experimental contexts, it's commonly used with:
Flow Cytometry Panel ComponentsWhen used in flow cytometry applications, M5/49.4.1 (whether FITC or PE-conjugated) is typically part of multi-color panels that may include other cell surface markers for comprehensive immune cell phenotyping. The antibody's ability to identify CD90-positive cells makes it valuable for distinguishing T cells, stem cells, and fibroblasts in mixed cell populations. The versatility of M5/49.4.1 in both functional depletion studies and analytical applications makes it a cornerstone reagent in mouse immunology research, often serving as either the primary depleting agent or as part of more complex experimental protocols involving multiple immune cell types and pathways. The clone M5/49.4.1 is a monoclonal antibody that targets Thy-1 (CD90) in mice. Thy-1 is a GPI-anchored protein belonging to the Ig superfamily, expressed on various cell types including thymocytes, peripheral T cells, myoblasts, epidermal cells, and keratinocytes. This antibody is often used for the depletion of T lymphocytes and in research related to cell adhesion, apoptosis, metastasis, inflammation, and fibrosis. Here are some key findings and uses of the M5/49.4.1 antibody in scientific literature: Key Uses and Findings
Overall, the M5/49.4.1 antibody is a tool for studying immune cell biology, particularly in the context of T cell functions and antigen presentation processes. References & Citations1 Bradley JE, Ramirez G, Hagood JS. Biofactors. 35(3):258-265. 2009. 2 Haeryfar SM, Hoskin DW. J Immunol. 173(6):3581-3588. 2004. 3 Rege TA, Hagood JS. FASEB J. 20(8):1045-1054. 2006. 4 Davignon D, Martz E, Reynolds T, et al. Proc Natl Acad Sci U S A. 78(7):4535-4539. 1981. 5 Weng X, Liao CM, Bagchi S, et al. Eur J Immunol. 44(12):3646-3657. 2014. Technical ProtocolsDepletion FA  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
