Anti-Mouse Jagged 2 – Purified in vivo GOLD™ Functional Grade

Clone HMJ2-1 is a monoclonal antibody that specifically recognizes mouse Jagged2 (JAG2), a ligand for the Notch signaling pathway. The most common in vivo applications of clone HMJ2-1 in mice are still limited and primarily focused on research use, with well-established applications in flow cytometry and mechanistic studies of the Notch pathway—but little direct evidence from the search results of routine use for direct in vivo administration in mice.

Primary Reported Applications

• Flow cytometric quantification and phenotyping: The manufacturer explicitly states that clone HMJ2-1 is used for flow cytometric analysis of murine cells, particularly to assess Jagged2 expression in immune cell populations such as splenocytes. This is considered an ex vivo application, as cells are harvested from mice, stained, and analyzed.
• Studying Notch signaling: Since Jagged2 is a key Notch ligand, HMJ2-1 has been used for mechanistic studies to evaluate Notch pathway involvement in immune cell differentiation and function, especially in transfected cell models or for detecting protein expression.

Potential and Investigational In Vivo Applications

• Functional Notch pathway interrogation: There is evidence (e.g., from studies cited in product literature) that some anti-Jagged2 antibodies may be used to modulate the Notch pathway, for example, to promote regulatory T cell differentiation or alter Jagged2 signaling in vitro or in cell transfer models. However, explicit direct in vivo administration (such as blocking or activating Jagged2 in live mice) with HMJ2-1 is not reported in the search results.

Limitations and Research-Only Use

• Lack of validated in vivo functional use: Product datasheets and vendor information repeatedly note that HMJ2-1 is intended for research use only, with no claims for therapeutic application or routine in vivo functional testing in mice.

Indirect or Related Uses

• Identification of Jagged2-expressing cells: By using HMJ2-1 after cell isolation from mice, researchers can phenotype or sort murine cells based on Jagged2 surface expression. This can be foundational for subsequent cell transfer or functional assays.
• Mechanistic studies with other Notch ligands: Notably, in vivo blocking antibodies against Jagged1 have been used in experimental autoimmune encephalomyelitis (EAE), but such usage is not documented for anti-Jagged2 clone HMJ2-1 in the provided results.

Summary Table

In summary: The most common applications of clone HMJ2-1 in mouse studies are detection and quantification of Jagged2 expression by flow cytometry and protein analysis, with no current standard validated use for direct in vivo administration or functional modulation in mice according to available sources.

Based on the available search results, specific information about antibodies or proteins commonly used in combination with HMJ2-1 in published literature is limited. The search results primarily focus on the characteristics and applications of HMJ2-1 itself rather than its use alongside other antibodies in experimental contexts.

What We Know About HMJ2-1

HMJ2-1 is an Armenian hamster monoclonal antibody that specifically targets mouse Jagged-2, a type I transmembrane protein that serves as a Notch receptor ligand. The antibody has been validated for several applications including flow cytometry, ELISA, and as an agonist that promotes Treg cell differentiation and enhances Jagged-2 Notch signaling via activation of RBP-Jk.

Limited Information on Co-usage

The search results do not provide detailed information about other antibodies or proteins routinely paired with HMJ2-1 in multi-marker studies or combination experiments. While the results mention that HMJ2-1 has been used in various research contexts involving the Notch signaling pathway, they do not specify which other antibodies investigators typically employ alongside it for co-staining, co-immunoprecipitation, or other combinatorial approaches.

To identify commonly co-used antibodies or proteins with HMJ2-1, you would need to review primary research articles that cite the use of this antibody in Notch signaling studies, T cell research, or developmental biology investigations where multiple markers are typically analyzed simultaneously.

Clone HMJ2-1 is a monoclonal antibody widely cited in scientific literature for its specificity in detecting Jagged2, a ligand in the Notch signaling pathway. The antibody is principally used in mouse models for applications such as flow cytometry, ELISA, and inhibition assays.

Key findings and details from scientific literature referencing clone HMJ2-1:

• Specificity and Use: HMJ2-1 specifically binds to the extracellular domain of mouse Jagged2, making it valuable for identifying and isolating cells expressing this protein.
• Research Applications: The antibody is used for characterizing cell populations involved in Notch signaling, which plays essential roles in immune cell differentiation, hematopoiesis, and development.
• Mechanistic Studies: Studies using HMJ2-1 have demonstrated that Jagged2-mediated Notch signaling can modulate immune responses, such as dendritic cell-mediated activation of natural killer (NK) cells.
• Assay Validation: The antibody is validated for multiple applications, especially flow cytometry and ELISA, which are standard techniques for quantitative and qualitative protein analysis in cell and tissue samples.
• Species Reactivity: While HMJ2-1 primarily targets mouse Jagged2, some sources indicate cross-reactivity with human and rat proteins, broadening its utility in comparative studies.

The literature underscores HMJ2-1's importance for research involving Notch pathway biology, specifically for dissecting Jagged2's role in cell signaling and immune modulation.

No major conflicting findings were reported, but direct source citations of HMJ2-1 in primary research typically focus on its use as a detection and characterization tool rather than as a direct modulator of function.

Based on the available search results, there is no specific information about dosing regimens for clone HMJ2-1 across different mouse models. Clone HMJ2-1 is identified as an Armenian Hamster monoclonal antibody that specifically detects mouse Jagged2, but the search results do not provide details about its dosing protocols, administration routes, or treatment schedules in experimental settings.

While the search results contain extensive information about dosing regimens for other antibodies used in mouse models—particularly checkpoint blockade antibodies like anti-PD-1 (200-500 μg per mouse) and anti-CTLA-4 (100-250 μg per mouse)—no comparable data is available for HMJ2-1. The search results explicitly note that dosing regimens for clone HMJ2-1 in different mouse models are not documented in the provided sources.

To obtain accurate dosing information for HMJ2-1, researchers would need to consult the original research publications that have used this antibody in vivo, contact the antibody manufacturer directly, or establish dosing parameters through pilot studies based on the antibody's binding characteristics and the specific experimental objectives.