Anti-Mouse LAG-3-BX033 (C9B7W); Cy5-RX033

Pricing & Details

Product No.L310
Clone
C9B7W
Protein
LAG-3
CD223
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD223, LAG3
Isotype
Rat
IgG1 κ
Applications
CODEX®
,
IHC FF
Prod No.
Size
Price
Avail.
Qty
Add to cart
L310-25 Tests
25 tests
$400.00
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CODEX® Details

Barcode
BX033
Reporter
Cy5-RX033
Fluorescent Dye
Cy5 is in the bright red fluorescent channel with an excitation max of 649 nm and an emission max of 666 nm.
Tissue Screened
MCA Sarcoma (Tumor)
Tissue Preparation
FF (Fresh Frozen)

Antibody and Reporter Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Murine CD223-Ig fusion protein
Formulation
This CODEX® barcoded antibody is formulated in phosphate buffered saline (150 mM NaCl) PBS, EDTA pH 7.2 containing 0.09% sodium azide as a preservative. The CODEX® reporter is lyophilized and formulated in 1X Tris-EDTA (TE) pH 8.0 (10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0)
Product Preparation
Manufactured in an animal-free facility using only in vitro cell culture techniques, purified by affinity chromatography, and conjugated to a specific barcode under optimal conditions.
State of Matter
CODEX® barcoded antibody - Liquid; CODEX® reporter - Lyophilized
Storage and Handling
This CODEX® barcoded antibody is stable when stored at 2-8°C for up to 1 year (do not freeze). The CODEX® reporter is stable when frozen at -20°C for up to 1 year.
Applications and Recommended Usage?
Quality Tested by Leinco
CODEX®; IHC FF - The suggested dilution for staining tissue in immunohistochemistry - Fresh Frozen (FF) is 1 μl of Anti-Mouse LAG-3 (BX033) in a final volume of 200 μl of CODEX® staining buffer.
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone C9B7W recognizes and specifically binds to an epitope in the D2 domain of CD223.
Antigen Distribution
CD223 is expressed on T regulatory cells, activated T cells and NK cells.
Background
Antibody LAG-3 is a 70-kD, type-I transmembrane glycoprotein within the Ig superfamily with four extracellular Ig-like domains (D1 to D4) and is structurally homologous to CD4. LAG-3 is a cell surface molecule with various biologic effects on T cell function. It has been reported to be involved in Treg suppressive function. It negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar manner to CTLA-4 and PD-1. Human LAG-3 is approximately 70% homologous with murine LAG3, and it binds MHC class II molecules with higher affinity than CD4. As an immune checkpoint receptor, LAG-3 is the target of various drug development programs seeking to expand treatments for cancer and autoimmune disorders. In its soluble form, LAG-3 is being developed as a cancer drug. As an antagonist, LAG-3 antibody can activate T effector cells via the downregulation of the LAG-3 inhibiting signal into pre-activated LAG-3+ cells. In addition, it can inhibit antigen-specific Treg suppressive activity. As an agonist antibody, it can be used to diminish an autoimmune response and is currently being investigated for the treatment of plaque psoriasis.

CODEX® reporters are made up of a fluorescent dye and a short oligonucleotide DNA barcode called a CODEX® Tag. Fluorescent reporters enable highly specific detection of corresponding barcodes, and the use of spectrally separated dyes allow for precise signal detection in up to three distinct fluorescence channels at one time.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
IHC

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.