Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade

Clone 15-3-1S is a monoclonal antibody that specifically binds to mouse MHC Class I alloantigens H-2Kk and H-2Dk, and it is commonly used in in vivo mouse studies to target these molecules.

In vivo applications for the 15-3-1S monoclonal antibody include:

• Blocking or depleting MHC Class I function: By binding to H-2Kk and H-2Dk on nucleated mouse cells, this antibody can block the interaction between MHC Class I molecules and CD8+ T cells, or even facilitate depletion of particular cell populations expressing these MHC variants in certain experimental models.
• Flow cytometry and cell sorting: The antibody can be used to label and identify cells expressing these specific MHC Class I variants during flow cytometry, allowing precise profiling of immune cell subsets in genetically defined mouse strains (generally H-2k haplotype strains, such as C3H).
• Immune modulation: Because MHC Class I molecules are critical for antigen presentation to CD8+ T cells, the in vivo administration of 15-3-1S can be used to study cytotoxic T cell responses or immune rejection phenomena in transplantation and immunological tolerance studies.

The antibody is purified, endotoxin-controlled, and formulated for safe injection in mouse models, making it a standard reagent for studies requiring in vivo manipulation or identification of H-2Kk and H-2Dk expressing cells.

In summary, 15-3-1S is mainly used in vivo to block, deplete, or identify cells expressing mouse MHC Class I H-2Kk/H-2Dk, enabling targeted investigation of immune mechanisms reliant on these molecules.

I don't have specific information about the storage temperature for the sterile packaged clone 15-3-1S in the provided search results. The search results contain information about NEBuilder HiFi DNA Assembly kits and ZAP-cDNA synthesis kits, but they don't mention the specific clone 15-3-1S you're asking about.

To provide accurate storage information for clone 15-3-1S, I would need access to the specific product documentation, manufacturer's instructions, or technical data sheet for that particular clone. Different biological materials have varying storage requirements depending on their nature, preparation method, and intended use.

If you have access to the original packaging or documentation for clone 15-3-1S, I'd recommend checking those materials for the manufacturer's storage recommendations, as proper storage conditions are critical for maintaining the viability and integrity of biological clones.

Commonly used antibodies or proteins studied with 15-3-1S (anti-CA 15-3) in the literature include:

• Antibodies against MUC1 (CA15.3) itself, notably monoclonal mouse IgG, polyclonal IgY, or IgG antibodies specific to unique peptide epitopes of MUC1/CA 15-3.
• Anti-DF3 antibodies, since the DF3 antigen is another designation for MUC1/CA15-3 in biomarker studies.
• Secondary detection antibodies such as labeled anti-human IgG are used for quantifying anti-CA15.3/MUC1 autoantibodies in patient serum.

Key context:

• MUC1 and CA 15-3: CA 15-3 is the commonly referenced circulating epitope of the MUC1 protein, a transmembrane glycoprotein widely studied in breast cancer. Various antibodies targeting different regions of MUC1 (including overlapping regions with CA 15-3) are frequently used together in both research and clinical immunoassays.
• Specific epitopes/peptides: Antibodies targeting specific regions such as amino acids 1066–1085 and 1085–1103 of MUC1 are mentioned as commonly paired with broader anti-CA 15-3 antibodies, allowing more granular investigation of immune recognition.
• Antibody types: Research frequently compares different sources and types of antibodies (e.g., IgY from egg yolk versus monoclonal mouse IgG) for anti-CA 15-3 immunoassays.
• Controls and isotypes: Control antibodies (irrelevant IgG or preimmune serum), anti-BSA, anti-KLH, and other isotype-matched antibodies are also commonly used for specificity assessment.

Summary table of commonly paired targets:

In summary, antibodies against MUC1/CA15.3's different epitopes, anti-DF3, and detection antibodies against human IgG are the primary reagents used in combination or parallel with 15-3-1S for characterization, assay development, and biomarker studies in the literature.

I was unable to find specific scientific literature citations or research findings related to "clone 15-3-1S" in the provided search results. The search results contain information about several different types of clones used in various research contexts, but none specifically mention clone 15-3-1S.

The search results do discuss other clones in scientific research, including:

HL-60 Clone 15 (HC15) Cell Line: This is a well-characterized cell line that can be differentiated into eosinophil-like cells for leukocyte migration research. However, this appears to be different from the clone 15-3-1S you're asking about, as it's referred to simply as "clone 15" or "HC15" in the context of HL-60 cells.

Tumor Clone Analysis Methods: The results include discussion of computational methods for inferring clone phylogenies from tumor samples, but these don't reference clone 15-3-1S specifically.

Bacterial Clones: There's mention of high-risk Enterobacterales clones like ST11, ST258, and ST15 in hospital settings, but again no reference to clone 15-3-1S.

Without access to search results that specifically mention clone 15-3-1S, I cannot provide the key findings from its citations in scientific literature. You may need to search more specifically for this particular clone designation, or it's possible that this clone hasn't been widely published about in the scientific literature, or the specific nomenclature "clone 15-3-1S" may be used in a very specialized research context that wasn't captured in these search results.