Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade

Clone 15-3-1S is a monoclonal antibody commonly used in mice for in vivo blockade of MHC class I molecules (specifically H-2Kk and H-2Dk). Its most established application is preventing islet allograft rejection in diabetic mice models by inhibiting immune recognition and attack on transplanted tissues expressing these MHC class I alloantigens.

Essential context and supporting details:

• Target specificity: Clone 15-3-1S binds to the mouse MHC class I molecules H-2Kk and H-2Dk, making it valuable for studies in C3H and other mouse strains with these haplotypes.
• Allograft rejection studies: Blocking MHC class I with this antibody is used to investigate mechanisms of immune-mediated graft rejection and tolerance, particularly in transplantation immunology.
• In vivo use: The antibody is specifically designated for in vivo administration and is purified for low endotoxin levels, suitable for studies requiring immunomodulation in living animals.

Additional relevant information:

• Researchers may also utilize clone 15-3-1S to study broader aspects of immune regulation, such as the role of MHC class I in antigen presentation and T cell activation.
• While the main application cited is in transplantation models (islet allografts), similar MHC class I blockade strategies can be adapted for other tissue transplant or immune modulation studies in mice expressing the H-2Kk or H-2Dk haplotypes.
• The immunogen for this antibody is C3H mouse spleen cells, confirming its specificity for certain mouse strains.

No search results indicate alternative or conflicting in vivo applications for clone 15-3-1S beyond MHC class I blockade and allograft research.

In the context of antibody-protein interactions, particularly those involving the CA15-3 antigen (which is a part of the MUC1 protein), several other antibodies and proteins are commonly used in research. However, the specific reference to "15-3-1S" is unclear, and it seems there might be a typographical error or confusion with "CA15-3," which is a widely studied antigen. Assuming the focus is on CA15-3, here are some commonly used antibodies and related proteins:

1. DF3 Antibody: This monoclonal antibody targets the MUC1 protein, which is associated with CA15-3. It's used in assays to detect glycovariants of CA15-3, such as neu5Gc-CA15-3.

2. Anti-MUC1 Antibodies: These are used to detect MUC1, which is overexpressed in various cancers. Anti-MUC1 antibodies like the rabbit polyclonal antibody used for immunoprecipitation are common in research.

3. Lectins: In antibody-lectin sandwich assays for CA15-3, lectins are used to detect specific glycosylation patterns on the CA15-3 antigen. Concanavalin A (ConA) is an example of a lectin used in such assays.

4. CA15-3 Capture Assays: These assays often involve monoclonal antibodies like DF3 and lectins to capture and detect specific glycoforms of CA15-3, enhancing diagnostic accuracy for breast cancer.

If "15-3-1S" refers to a different entity, please provide more context or clarify its meaning to assist in identifying the relevant antibodies or proteins.

The key findings from scientific literature referring to "clone 15-3-1S" specifically could not be identified in the provided search results. No direct references or citations mention "clone 15-3-1S," and none of the indexed articles explicitly discuss findings related to a clone with this designation. Several results discuss similarly named clones (such as HL-60 clone 15 or high-risk ST15 bacterial clones), but these are not clearly the same as "15-3-1S".

If you are referring to a specific clone used in molecular biology, immunology (e.g., a monoclonal antibody clone), or microbial research, please clarify the organism or context. This will allow for targeted identification of relevant key findings. Based on current search results, "clone 15-3-1S" does not have cited findings in prominent peer-reviewed literature.

If you meant HL-60 clone 15:

• This cell line is used as a model for differentiating into eosinophil-like cells.
• It is functionally assessed through proteomics, migration assays, and other immune-relevant assays to model leukocyte behavior and is considered a tool in eosinophil research.

If you meant ST15 (a high-risk Klebsiella pneumoniae clone):

• It is commonly associated with ESBL (extended-spectrum beta-lactamase) production, particularly CTX-M-15, and plays a role in antimicrobial resistance dissemination.

For clone 15-3-1S, please provide more detail for a more accurate synthesis. Current scientific literature indexed in the provided results does not contain key findings for this specific clone.

There are no dosing data for clone 15-3-1S in the provided search results, nor is this clone specifically mentioned in the sources. Therefore, there is insufficient information to determine how dosing regimens of clone 15-3-1S vary across different mouse models from the given data.

Relevant context about dosing regimens in mouse models:

• Dosing regimens for monoclonal antibodies and other biologics in mice often depend on variables such as mouse strain, immunogenicity, route of administration, and the experimental objective.
• For humanized antibodies, certain mouse strains (e.g., those engineered to tolerate human IgG1) may allow for higher and repeated dosing without developing strong anti-human responses, whereas wild-type mice may rapidly clear antibodies due to immune reactions after 2–3 weeks.
• For other antibody clones and biologics, common dosing strategies include single or repeated doses ranging from 2.5 μg/mouse for cytokines to 200 μg/mouse for some antibodies, with intervals from daily to every several days depending on purpose and tolerability.
• Switching between mouse models (e.g., immunocompetent vs. immunodeficient or humanized strains) typically requires adjusting dose frequency, amount, and observation for immune responses.

If you are seeking information on clone 15-3-1S specifically:

• Please provide additional context (e.g., target antigen, isotype, application) or clarify whether this is a custom or less-common reagent.
• Citing experimental protocols or product datasheets for this antibody from relevant suppliers or publications might yield the specific dosing regimens used.

In summary, without direct data on clone 15-3-1S, only general principles can be provided: dosing usually requires optimization for the particular mouse strain and intended experimental outcome, with repeated administration protocols typically modified for tolerance and target engagement. If more information about clone 15-3-1S is available, supplying it may allow for a more targeted search and answer.