Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade
Product No.: H466
Clone 15-3-1S Target MHC Class I (H-2Kk, H-2Dk) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Isotype Mouse IgG2a k Applications B , FA , FC , IF , RIA |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Immunogen C3H spleen cells Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? B, FA, FC, IF, RIA Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity 15-3-1S activity is directed against MHC Class I H-2Kk and H-2Dk. 15-3-1S cross
reacts with the H-2r haplotype. Background H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell. H-2Kk and H-2Dk are MHC class I proteins originally
identified in the C3H mouse2. 15-3-1S was generated by immunizing C3H mice with spleen cells from C3H mice3. Subsequently, spleen cells from the immunized mice were fused with Sp2/0-Ag14 myeloma cells to create hybridoma lines. 15-3-1S was characterized by performing cytotoxic titer of hybridoma ascites on a panel of H-2 haplotypes4. Additionally, blocking ability and specificity were analyzed. 15-3-1S has relatively low affinity for H-2Kk according to radioactive binding assays with B10.A spleen cells. Antigen Distribution H-2Kk is present on 100% of T cells, B cells, erythrocytes, macrophages,
cells of endocrine origin including B cells of Langerhans as well as endothelial and epithelial
tissues. H-2Dk is expressed on nucleated cells from mice of the H-2Dk haplotype. Ligand/Receptor CD3/TCR, CD8 Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 15-3-1S is a monoclonal antibody that specifically binds to mouse MHC Class I alloantigens H-2Kk and H-2Dk, and it is commonly used in in vivo mouse studies to target these molecules. In vivo applications for the 15-3-1S monoclonal antibody include:
The antibody is purified, endotoxin-controlled, and formulated for safe injection in mouse models, making it a standard reagent for studies requiring in vivo manipulation or identification of H-2Kk and H-2Dk expressing cells. In summary, 15-3-1S is mainly used in vivo to block, deplete, or identify cells expressing mouse MHC Class I H-2Kk/H-2Dk, enabling targeted investigation of immune mechanisms reliant on these molecules. I don't have specific information about the storage temperature for the sterile packaged clone 15-3-1S in the provided search results. The search results contain information about NEBuilder HiFi DNA Assembly kits and ZAP-cDNA synthesis kits, but they don't mention the specific clone 15-3-1S you're asking about. To provide accurate storage information for clone 15-3-1S, I would need access to the specific product documentation, manufacturer's instructions, or technical data sheet for that particular clone. Different biological materials have varying storage requirements depending on their nature, preparation method, and intended use. If you have access to the original packaging or documentation for clone 15-3-1S, I'd recommend checking those materials for the manufacturer's storage recommendations, as proper storage conditions are critical for maintaining the viability and integrity of biological clones. Commonly used antibodies or proteins studied with 15-3-1S (anti-CA 15-3) in the literature include:
Key context:
Summary table of commonly paired targets:
In summary, antibodies against MUC1/CA15.3's different epitopes, anti-DF3, and detection antibodies against human IgG are the primary reagents used in combination or parallel with 15-3-1S for characterization, assay development, and biomarker studies in the literature. I was unable to find specific scientific literature citations or research findings related to "clone 15-3-1S" in the provided search results. The search results contain information about several different types of clones used in various research contexts, but none specifically mention clone 15-3-1S. The search results do discuss other clones in scientific research, including: HL-60 Clone 15 (HC15) Cell Line: This is a well-characterized cell line that can be differentiated into eosinophil-like cells for leukocyte migration research. However, this appears to be different from the clone 15-3-1S you're asking about, as it's referred to simply as "clone 15" or "HC15" in the context of HL-60 cells. Tumor Clone Analysis Methods: The results include discussion of computational methods for inferring clone phylogenies from tumor samples, but these don't reference clone 15-3-1S specifically. Bacterial Clones: There's mention of high-risk Enterobacterales clones like ST11, ST258, and ST15 in hospital settings, but again no reference to clone 15-3-1S. Without access to search results that specifically mention clone 15-3-1S, I cannot provide the key findings from its citations in scientific literature. You may need to search more specifically for this particular clone designation, or it's possible that this clone hasn't been widely published about in the scientific literature, or the specific nomenclature "clone 15-3-1S" may be used in a very specialized research context that wasn't captured in these search results. References & Citations1 Yoshida R. Adv Immunol. 124:207-247. 2014. 2 Watts S, Vogel JM, Harriman WD, et al. J Immunol. 139(11):3878-3885. 1987. 3 Ozato K, Mayer N, Sachs DH. J Immunol. 124(2):533-540. 1980. 4 Epstein SL, Ozato K, Sachs DH. J Immunol. 125(1):129-135. 1980. 5 Lenz A, Heufler C, Rammensee HG, et al. Proc Natl Acad Sci U S A. 86(19):7527-7531. 1989. 6 Caughman SW, Sharrow SO, Shimada S, et al. Proc Natl Acad Sci U S A. 83(19):7438-7442.1986. 7 Madsen JC, Superina RA, Wood KJ, et al. Nature. 332(6160):161-164. 1988. Technical ProtocolsCertificate of Analysis |
Formats Available
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H465 | |
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