Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade

Product No.: H466

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Clone
15-3-1S
Target
MHC Class I (H-2Kk, H-2Dk)
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Isotype
Mouse IgG2a k
Applications
B
,
FA
,
FC
,
IF
,
RIA

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Mouse
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
C3H spleen cells
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
B,
FA,
FC,
IF,
RIA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
15-3-1S activity is directed against MHC Class I H-2Kk and H-2Dk. 15-3-1S cross reacts with the H-2r haplotype.
Background
H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response1. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell. H-2Kk and H-2Dk are MHC class I proteins originally identified in the C3H mouse2.

15-3-1S was generated by immunizing C3H mice with spleen cells from C3H mice3. Subsequently, spleen cells from the immunized mice were fused with Sp2/0-Ag14 myeloma cells to create hybridoma lines. 15-3-1S was characterized by performing cytotoxic titer of hybridoma ascites on a panel of H-2 haplotypes4. Additionally, blocking ability and specificity were analyzed. 15-3-1S has relatively low affinity for H-2Kk according to radioactive binding assays with B10.A spleen cells.

Antigen Distribution
H-2Kk is present on 100% of T cells, B cells, erythrocytes, macrophages, cells of endocrine origin including B cells of Langerhans as well as endothelial and epithelial tissues. H-2Dk is expressed on nucleated cells from mice of the H-2Dk haplotype.
Ligand/Receptor
CD3/TCR, CD8
Research Area
Immunology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone 15-3-1S is a monoclonal antibody that specifically binds to mouse MHC Class I alloantigens H-2Kk and H-2Dk, and it is commonly used in in vivo mouse studies to target these molecules.

In vivo applications for the 15-3-1S monoclonal antibody include:

  • Blocking or depleting MHC Class I function: By binding to H-2Kk and H-2Dk on nucleated mouse cells, this antibody can block the interaction between MHC Class I molecules and CD8+ T cells, or even facilitate depletion of particular cell populations expressing these MHC variants in certain experimental models.
  • Flow cytometry and cell sorting: The antibody can be used to label and identify cells expressing these specific MHC Class I variants during flow cytometry, allowing precise profiling of immune cell subsets in genetically defined mouse strains (generally H-2k haplotype strains, such as C3H).
  • Immune modulation: Because MHC Class I molecules are critical for antigen presentation to CD8+ T cells, the in vivo administration of 15-3-1S can be used to study cytotoxic T cell responses or immune rejection phenomena in transplantation and immunological tolerance studies.

The antibody is purified, endotoxin-controlled, and formulated for safe injection in mouse models, making it a standard reagent for studies requiring in vivo manipulation or identification of H-2Kk and H-2Dk expressing cells.

In summary, 15-3-1S is mainly used in vivo to block, deplete, or identify cells expressing mouse MHC Class I H-2Kk/H-2Dk, enabling targeted investigation of immune mechanisms reliant on these molecules.

I don't have specific information about the storage temperature for the sterile packaged clone 15-3-1S in the provided search results. The search results contain information about NEBuilder HiFi DNA Assembly kits and ZAP-cDNA synthesis kits, but they don't mention the specific clone 15-3-1S you're asking about.

To provide accurate storage information for clone 15-3-1S, I would need access to the specific product documentation, manufacturer's instructions, or technical data sheet for that particular clone. Different biological materials have varying storage requirements depending on their nature, preparation method, and intended use.

If you have access to the original packaging or documentation for clone 15-3-1S, I'd recommend checking those materials for the manufacturer's storage recommendations, as proper storage conditions are critical for maintaining the viability and integrity of biological clones.

Commonly used antibodies or proteins studied with 15-3-1S (anti-CA 15-3) in the literature include:

  • Antibodies against MUC1 (CA15.3) itself, notably monoclonal mouse IgG, polyclonal IgY, or IgG antibodies specific to unique peptide epitopes of MUC1/CA 15-3.
  • Anti-DF3 antibodies, since the DF3 antigen is another designation for MUC1/CA15-3 in biomarker studies.
  • Secondary detection antibodies such as labeled anti-human IgG are used for quantifying anti-CA15.3/MUC1 autoantibodies in patient serum.

Key context:

  • MUC1 and CA 15-3: CA 15-3 is the commonly referenced circulating epitope of the MUC1 protein, a transmembrane glycoprotein widely studied in breast cancer. Various antibodies targeting different regions of MUC1 (including overlapping regions with CA 15-3) are frequently used together in both research and clinical immunoassays.
  • Specific epitopes/peptides: Antibodies targeting specific regions such as amino acids 1066–1085 and 1085–1103 of MUC1 are mentioned as commonly paired with broader anti-CA 15-3 antibodies, allowing more granular investigation of immune recognition.
  • Antibody types: Research frequently compares different sources and types of antibodies (e.g., IgY from egg yolk versus monoclonal mouse IgG) for anti-CA 15-3 immunoassays.
  • Controls and isotypes: Control antibodies (irrelevant IgG or preimmune serum), anti-BSA, anti-KLH, and other isotype-matched antibodies are also commonly used for specificity assessment.

Summary table of commonly paired targets:

TargetNotes/Purpose
MUC1 (various regions)Detect total antigen or specific epitopes; MUC1 is the parent protein for CA 15-3
DF3 antigenAlternative name for MUC1/CA 15-3 as used in some studies
Anti-human IgGDetects patient antibodies against MUC1/CA 15-3 in assays
Control proteinsBSA, KLH used as conjugates or controls

In summary, antibodies against MUC1/CA15.3's different epitopes, anti-DF3, and detection antibodies against human IgG are the primary reagents used in combination or parallel with 15-3-1S for characterization, assay development, and biomarker studies in the literature.

I was unable to find specific scientific literature citations or research findings related to "clone 15-3-1S" in the provided search results. The search results contain information about several different types of clones used in various research contexts, but none specifically mention clone 15-3-1S.

The search results do discuss other clones in scientific research, including:

HL-60 Clone 15 (HC15) Cell Line: This is a well-characterized cell line that can be differentiated into eosinophil-like cells for leukocyte migration research. However, this appears to be different from the clone 15-3-1S you're asking about, as it's referred to simply as "clone 15" or "HC15" in the context of HL-60 cells.

Tumor Clone Analysis Methods: The results include discussion of computational methods for inferring clone phylogenies from tumor samples, but these don't reference clone 15-3-1S specifically.

Bacterial Clones: There's mention of high-risk Enterobacterales clones like ST11, ST258, and ST15 in hospital settings, but again no reference to clone 15-3-1S.

Without access to search results that specifically mention clone 15-3-1S, I cannot provide the key findings from its citations in scientific literature. You may need to search more specifically for this particular clone designation, or it's possible that this clone hasn't been widely published about in the scientific literature, or the specific nomenclature "clone 15-3-1S" may be used in a very specialized research context that wasn't captured in these search results.

References & Citations

1 Yoshida R. Adv Immunol. 124:207-247. 2014.
2 Watts S, Vogel JM, Harriman WD, et al. J Immunol. 139(11):3878-3885. 1987.
3 Ozato K, Mayer N, Sachs DH. J Immunol. 124(2):533-540. 1980.
4 Epstein SL, Ozato K, Sachs DH. J Immunol. 125(1):129-135. 1980.
5 Lenz A, Heufler C, Rammensee HG, et al. Proc Natl Acad Sci U S A. 86(19):7527-7531. 1989.
6 Caughman SW, Sharrow SO, Shimada S, et al. Proc Natl Acad Sci U S A. 83(19):7438-7442.1986.
7 Madsen JC, Superina RA, Wood KJ, et al. Nature. 332(6160):161-164. 1988.
B
FA
Flow Cytometry
IF
RIA

Certificate of Analysis

Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.