Anti-Mouse MHC Class I (H-2Kk, H-2Dk) (Clone 15-3-1S) – Purified in vivo PLATINUM™ Functional Grade

C3H spleen cells

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

15-3-1S activity is directed against MHC Class I H-2Kk and H-2Dk. 15-3-1S cross reacts with the H-2r haplotype.

H-2, the murine major histocompatibility complex (MHC), is composed of a diverse group of antigens divided into class I and II proteins that function in immune response¹. MHC class I molecules bind peptides generated by the degradation of cytosolic proteins, and then display those peptides on the cell surface. Generally, these peptides are derived from normal metabolism, but they can also be derived from foreign proteins during viral infection or allotransplantation. When peptides are recognized as foreign, cytotoxic T lymphocytes specific to the MHC class I-peptide complex kill the presenting cell. H-2Kk and H-2Dk are MHC class I proteins originally identified in the C3H mouse².

15-3-1S was generated by immunizing C3H mice with spleen cells from C3H mice³. Subsequently, spleen cells from the immunized mice were fused with Sp2/0-Ag14 myeloma cells to create hybridoma lines. 15-3-1S was characterized by performing cytotoxic titer of hybridoma ascites on a panel of H-2 haplotypes⁴. Additionally, blocking ability and specificity were analyzed. 15-3-1S has relatively low affinity for H-2Kk according to radioactive binding assays with B10.A spleen cells.

H-2Kk is present on 100% of T cells, B cells, erythrocytes, macrophages, cells of endocrine origin including B cells of Langerhans as well as endothelial and epithelial tissues. H-2Dk is expressed on nucleated cells from mice of the H-2Dk haplotype.

CD3/TCR, CD8

1 Yoshida R. Adv Immunol. 124:207-247. 2014.
2 Watts S, Vogel JM, Harriman WD, et al. J Immunol. 139(11):3878-3885. 1987.
3 Ozato K, Mayer N, Sachs DH. J Immunol. 124(2):533-540. 1980.
4 Epstein SL, Ozato K, Sachs DH. J Immunol. 125(1):129-135. 1980.
5 Lenz A, Heufler C, Rammensee HG, et al. Proc Natl Acad Sci U S A. 86(19):7527-7531. 1989.
6 Caughman SW, Sharrow SO, Shimada S, et al. Proc Natl Acad Sci U S A. 83(19):7438-7442.1986.
7 Madsen JC, Superina RA, Wood KJ, et al. Nature. 332(6160):161-164. 1988.