DAB Substrate Kit

Product No.: D100


Product Type Substrate and Assay Reagents Applications IHC , WB


Select Product Size 300 ml — $75.00 900 ml — $155.00	Qty Max: Min: 1 Step: 1 Select A Size ADD TO CART Login For mAb Advantage Pricing Contact Us for Bulk Quantities


Product Details Components The Kit contains the following reagents to make an active stable substrate solution for use with the enzyme horseradish peroxidase: 1. 12 ml Stable DAB Solution (Store below -20^oC) 2. 6 ml Peroxide Solution (Store 2-8^oC) 3. 6 ml Buffer Stock Solution (Store 2-8^oC) Storage and Handling Store the DAB (25X) stock solution below 0^oC. The remaining reagents are stored at 2-8^oC. Description Background DAB Substrate (3,3/-Diaminobenzidine) is the most commonly used substrate for demonstrating the presence of horseradish peroxidase in immunohistochemistry or immunoblotting. It produces a stable water/alcohol insoluble reddish-brown product. The slides may be dehydrated through alcohols to xylene and mounted with any commercially available mounting media. Directions for Use 1. To 5ml distilled water add 100µl (2 drops) of buffer stock solution. 2. Add 200µl (4 drops) of DAB stock solution. 3. Add 100µl (2 drops) of Hydrogen Peroxide solution. Mix well. (Working solution stable for at least 6 hours at room temperature.) Application For use as an insoluble substrate for horseradish peroxidase. This substrate solution is suitable for use in immunoblotting and immunohistological staining procedures. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Hazard Description Caution: DAB is a possible carcinogen. Avoid contact by using gloves and mask when handling. References 1. Nakane, P.K. and Pierce, G.B. (1967) J. Histochem. Cytochem. 14, 929 2. Barile, F. and Trombetta, L.D., (1982) J Histochem. 5:12 3. Rose, N.R., Friedman, H. and Fahey, J.L., eds. (1986) Manual of Clinical Laboratory Immunology, 2rd. ed. ASM, Washington, D.C. 4. Hsu, S. and Soban, E. (1982) J. Histochem. Cytochem. 30:1079 Related Protocols ⋅

Product Details

Components

The Kit contains the following reagents to make an active stable substrate solution for use with the enzyme horseradish peroxidase:

1. 12 ml Stable DAB Solution (Store below -20^oC)
2. 6 ml Peroxide Solution (Store 2-8^oC)
3. 6 ml Buffer Stock Solution (Store 2-8^oC)

Storage and Handling

Store the DAB (25X) stock solution below 0^oC. The remaining reagents are stored at 2-8^oC.

Description

Background

DAB Substrate (3,3/-Diaminobenzidine) is the most commonly used substrate for demonstrating the presence of horseradish peroxidase in immunohistochemistry or immunoblotting. It produces a stable water/alcohol insoluble reddish-brown product. The slides may be dehydrated through alcohols to xylene and mounted with any commercially available mounting media.

Directions for Use

1. To 5ml distilled water add 100µl (2 drops) of buffer stock solution.
2. Add 200µl (4 drops) of DAB stock solution.
3. Add 100µl (2 drops) of Hydrogen Peroxide solution. Mix well. (Working solution stable for at least 6 hours at room temperature.)

Application For use as an insoluble substrate for horseradish peroxidase. This substrate solution is suitable for use in immunoblotting and immunohistological staining procedures.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Hazard Description

Caution: DAB is a possible carcinogen. Avoid contact by using gloves and mask when handling.

References

1. Nakane, P.K. and Pierce, G.B. (1967) J. Histochem. Cytochem. 14, 929
2. Barile, F. and Trombetta, L.D., (1982) J Histochem. 5:12
3. Rose, N.R., Friedman, H. and Fahey, J.L., eds. (1986) Manual of Clinical Laboratory Immunology, 2rd. ed. ASM, Washington, D.C.
4. Hsu, S. and Soban, E. (1982) J. Histochem. Cytochem. 30:1079