Human Brain Tissue Lysate

Human Brain Tissue Lysate

Product No.: H1407

[product_table name="All Top" skus="H1407"]

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Product Type
Tissue Lysate

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Details

Tissue Type
Brain Tissue
Diagnosis
Normal
Preparation
Brain tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% β-mercaptoethanol.
Safety Testing
This material has been tested by accepted techniques and has been found to be negative for HBsAg, HIV 1/2, and HCV. Additional patient information may be available upon request.
Host
Human
Concentration
2.0 mg/ml
Formulation
1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% ß-mercaptoethanol.
Expiration Date
One year from date of receipt
Storage
For short term storage of this tissue lysate, store at 2-8°C. Avoid Repeated Freeze Thaw Cycles.</b> For long term storage freeze working aliquots at -70°C. Under proper storage conditions the shelf life of this tissue lysate is 6 months from the date of receipt.
Applications and Suggested Working Dilutions
This lysate is for use in Western blotting, 10 µg to 20 µg per lane is recommended for mini gel.
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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.