Mouse IgG2a Isotype Control [Clone C1.18.4] — Purified in vivo PLATINUM™ Functional Grade
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831654 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG2a isotype control is a monoclonal antibody and has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone C1.18.4, a mouse IgG2a isotype control antibody, is commonly used in in vivo applications for several purposes:
This antibody is often paired with antigen-specific IgG2a antibodies to provide a control for experiments, allowing researchers to distinguish between specific and non-specific binding effects. C1.18.4 is a mouse IgG2a isotype control antibody used in immunological experimentsprimarily as a negative control. In the literature, it is commonly used alongside antibodies targeting specific antigens—especially other mouse IgG2a antibodies that target surface markers or immune-related proteins—to control for non-specific binding effects. Commonly used antibodies or proteins with C1.18.4 include:
C1.18.4 itself is not used for detection of any specific cellular target but is co-applied as a control in experiments where specificity of mouse IgG2a-based detection or depletion antibodies may be in question. Its main role is to confirm that any observed effects are due to the target-specific antibody and not simply due to the IgG2a backbone or non-specific interactions. Key pairings in published research include:
In summary, anti-MHC class I antibodies (e.g., 34-1-2S, SF1.1.10, AF6-88.5) and other cell surface marker antibodies are the most common antibodies used alongside C1.18.4 isotype control in immunological research. Key Findings from Clone C1.18.4 Citations in Scientific LiteratureOverview of Clone C1.18.4 Primary Uses in Research
No Intrinsic Biological Findings Technical Specifications
Summary Table: Utility and Application
Key Studies Citing C1.18.4
Notable Functional Study (Contextual Example)A study showed that sialylated mouse IgG2a antibodies (using C1.18.4 as an irrelevant control) did not suppress anti-TNP antibody-mediated responses, reinforcing its role as a negative control rather than a functional modulator. ConclusionThe key scientific finding regarding clone C1.18.4 is its universal acceptance as a high-quality, minimal cross-reacting, isotype-matched negative control in mouse biomedical research. It is cited frequently not for discovery of biological mechanisms, but for ensuring rigor and validity in antibody-based assays. No direct citations relate to novel biological functions or therapeutic properties of the clone itself. Dosing regimens of clone C1.18.4 in mouse models are not standardized and typically vary depending on the design of the experimental study, as its main function is as an IgG2a isotype control to match the dose, route, and schedule of the test antibody in each specific model. Researchers generally follow these principles in dosing:
Key points:
In summary, C1.18.4 dosing regimens are variable and matched directly to the experimental antibody protocol rather than to mouse model type or characteristics. References & Citations1. Ikeda Y. et al. (2003) Blood.101(2):621-3. Article Link 2. Wright, TM et al. (2000) J Immunol.164(12):6138-46.PubMed 3. Wright, TM et al. (2001) Arthritis Rheum. 44(7):1654-9. PubMed 4. Nishikawa, Takeji et al. (2000) Journal of Investigative Dermatology 114(1): 88-94. PubMed Technical ProtocolsCertificate of Analysis |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
