Mouse IgG2a Isotype Control – Purified in vivo GOLD™ Functional Grade

Mouse IgG2a Isotype Control – Purified in vivo GOLD™ Functional Grade

Product No.: I-118

[product_table name="All Top" skus="I-118"]

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Prod. No.I-118
Clone
Antibody Type Monoclonal
Isotype
IgG2a

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Antibody Details

Product Details

Host Species
Mouse
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Applications and Recommended Usage?
Quality Tested by Leinco
FC This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Specificity
This Mouse IgG2a (Anti-KLH) isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. The isotype of this antibody is Mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissue

Antigen Details

References & Citations

1.) Ikeda Y. et al. (2003) Blood.101(2):621-3. Article Link
2.) Wright, TM et al. (2000) J Immunol.164(12):6138-46.PubMed
3.) Wright, TM et al. (2001) Arthritis Rheum. 44(7):1654-9. PubMed
4.) Nishikawa, Takeji et al. (2000) Journal of Investigative Dermatology 114(1): 88-94. PubMed
5.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link
Flow Cytometry
in vivo Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.