Mouse IgG2a Isotype Control – Purified In vivo GOLD™ Functional Grade

Pricing & Details

  
Clone
C1.18.4
Antibody Type
Isotype Control
Isotype
Mouse
IgG2a
Prod No.
Size
Price
Avail.
Qty
Add to cart
I-118-1.0 mg
1.0 mg
$95.00
In stock
I-118-5.0 mg
5.0 mg
$298.00
In stock
I-118-25 mg
25 mg
$775.00
In stock
I-118-50 mg
50 mg
$1,275.00
In stock
I-118-100 mg
100 mg
$1,998.00
In stock
  
Host Species
Mouse
Product Concentration
≥ 2.0 mg/ml
Endotoxin Level
≤ 1.0 EU/mg as determined by the LAL method
Purity
>95% by SDS-PAGE and analyzed by silver stain.
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (PBS) pH 7.2 - 7.4, 150 mM NaCl with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Applications and Recommended Usage ?
(Quality Tested by Leinco)
Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing CD11a. A suggested method would be to stain cells expressing CD11a with 1 µg per 1 x 106 cells in a total staining volume of 100 µl followed by PN:A104.
Specificity
This Mouse IgG2a (Anti-KLH) isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. The isotype of this antibody is Mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissue

References & Citations

1. Ikeda Y. et al. (2003) Blood.101(2):621-3. Article Link
2. Wright, TM et al. (2000) J Immunol.164(12):6138-46.PubMed
3. Wright, TM et al. (2001) Arthritis Rheum. 44(7):1654-9. PubMed
4. Nishikawa, Takeji et al. (2000) Journal of Investigative Dermatology 114(1): 88-94. PubMed

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.