Recombinant Human Angiopoietin-Like Protein 3

Recombinant Human Angiopoietin-Like Protein 3

Product No.: A324

[product_table name="All Top" skus="A324"]

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Alternate Names
Product Type
Recombinant Protein
Expression Host
sf Insect Cells

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Angiopoietin-like proteins ANGPTL3 and ANGPTL4 are secreted proteins mainly expressed in liver that have been demonstrated to regulate triglyceride metabolism by inhibiting the lipolysis of triglyceride-rich lipoproteins. ANGPTL3 is structurally similar to angiopoietins, which are vascular endothelial growth factors. The experimental results show that Angptl3 and Angptl4 function to regulate circulating triglyceride levels during different nutritional states and therefore play a role in lipid metabolism during feeding/fasting through differential inhibition of Lipoprotein lipase (LPL). g deletion mutants of human ANGPTL3, it was demonstrated that the N-terminal domain containing fragment - (17-207) and not the C-terminal fibrinogen-like domain containing fragment - (207-460) increased the plasma triglyceride levels in mice. The bold section of the amino acid sequence represents the His-tag.

Protein Details

>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Fusion Protein Tag
Histidine Tag
Protein Accession No.
Amino Acid Sequence
srid qdnssfdsls pepksrfaml ddvkilangl lqlghglkdf vhktkgqind ifqklnifdq sfydlslqts eikeeekelr rttyklqvkn eevknmslel nsklesllee killqqkvky leeqltnliq nqpetpehpe vtslktfvek qdnsikdllq tvedqykqln qqhsqikeie nqlrrtsiqe pteislsskp raprttpflq lneirnvkhd gipaecttiy nrgehtsgmy airpsnsqvf hvycdvisgs pwtliqhrid gsqnfnetwe nykygfgrld gefwlgleki ysivkqsnyv lrieledwkd nkhyieysfy lgnhetnytl hlvaitgnvp naipenkdlv fstwdhkakg hfncpegysg gwwwhdecge nnlngkynkp rakskperrr glswksqngr lysikstkml ihptdsesfe hhhhhhhhhh
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human ANGPTL3 is Mr 53.2 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 54-59 kDa.
Predicted Molecular Mass
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) and sodium chloride (NaCl) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Nishizawa, Y. et al. (2007) J Vasc Res. 44(1):61-6.
2. Zhang, XY. and Guan, YF. (2006) Sheng Li Ke Xue Jin Zhan. 37(2):159-61.
3. Shimomura, I. et al. (2004) Biochem Biophys Res Commun. 322(3):1080-5.
4. Shimomura, I. et al. (2003) J Biol Chem. 278(24):21344-51.
5. Shimomura, I. et al. (2003) Biochem Biophys Res Commun. 301(2):604-9.
6. Cai, TQ. et al. (2003) J Lipid Res. 44(1):136-43.
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.