Recombinant Human BMP-13

Bone Morphogenetic Protein-13 (BMP-13) is a member of the transforming growth factor beta (TGF-beta) superfamily of growth factors that may regulate patterning of the ectoderm by interacting with bone morphogenetic proteins and control eye development. The biological actions of BMP-13 remain poorly understood. Expression of BMP-13 is predominantly seen in hypertrophic chondrocytes in regions of endochondral bone formation during development. BMP-13 in post natal articular cartilage suggests that this protein may function in an autocrine/paracrine way to regulate the growth and maintenance of articular cartilage. In addition to this BMP-13 has been shown to induce neotendon/neoligament formation when injected on a collagen carrier subcutaneously or intramuscularly. This indicates that BMP-13 may have therapeutic value for tendon or ligament healing following injury.

At the amino acid sequence level, human and mouse GDF-6 are 99% identical. GDF-6 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activation of Smad proteins.

The ED₅₀ was determined by its ability to induce alkaline phosphatase production by ATDC-5 chondrogenic cells¹ in the range of 2.0-3.0 µg/ml.

P43028

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Thr335

Solution

The predicted molecular weight of Recombinant Human BMP-13 is 13.7 kDa.

13.7

This recombinant protein was 0.2 µm filtered and is supplied in a solution of 35% acetonitrile (CH₃CN) and 0.1% trifluoroacetic acid (TFA).

Working aliquots of this recombinant protein solution are stable for up to twelve months at -20°C to -70°C in a manual defrost freezer. Upon thawing, in the presence of a carrier protein, this recombinant protein can be stored at 2° - 8°C for one month without detectable loss of activity. For long-term storage, aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles.

Dry Ice

1. Nakamura, K. et al. (1999) Exp. Cell Res. 250:351