Recombinant Human Cathepsin L

Recombinant Human Cathepsin L

Product No.: C1363

[product_table name="All Top" skus="C1363"]

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Alternate Names
Ctsl1
Product Type
Recombinant Protein
Expression Host
NS0 Cells
Species
Human

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Background

A cathepsin is a cysteine or aspartic protease, a type of protein that breaks apart other proteins, found in many types of cells including those in all animals. There are approximately a dozen members of this family, which are distinguished by their structure and which proteins they cleave. Most of the members become activated at the low pH found in lysosomes. Thus, the activity of this family lies almost entirely within those organelles. Cathepsins have a vital role in mammalian cellular turnover, e.g. bone resorption. They degrade polypeptides and are distinguished by their substrate specificites.

Protein Details

Purity
>95% by SDS Page and analyzed by silver stain.
Endotoxin Level
<1.0 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human Cathepsin L was determined by its ability to cleave a fluorescent peptide substrate, N-carbobenzyloxy-Leu-Arg-7-amido-4-methylcoumarin (Z-L-R-AMC). The specific activity, measured with 10 μM Z-L-R-AMC and 1 ng enzyme in 100 μL of 50 mM MES, 5 mM dithiothreitol, pH 6.0, at room temperature, is > 15,000 pmoles/min/μg.
Fusion Protein Tag
Histidine Tag
Protein Accession No.
Amino Acid Sequence
eaprsvdwre kgyvtpvknq gqcgscwafs atgalegqmf rktgrlisls eqnlvdcsgp qgnegcnggl mdyafqyvqd nggldseesy pyeateesck ynpkysvand tgfvdipkqe kalmkavatv gpisvaidag hesflfykeg iyfepdcsse dmdhgvlvvg ygfestesdn nkywlvknsw geewgmggyv kmakdrrnhc giasaasypt vhhhhhh
N-terminal Sequence Analysis
Glu113 & Ala114
State of Matter
Frozen
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Cathepsin L is Mr 36 kDa.
Predicted Molecular Mass
36
Formulation
The cytokine is supplied frozen in 50 mM sodium acetate, 0.4 M NaCl, pH 5.0.
Storage and Stability
The frozen protein is stable for 6 months when kept a -20°C to -70°C in a manual defrost freezer. Avoid repeat freeze thaw cycles..
Country of Origin
USA
Shipping
Dry Ice
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.