Recombinant Human CCL14a/HCC-1

Chemokine (C-C motif) ligand 14 (CCL14), also known as HCC-1, is a small cytokine belonging to the CC chemokine family. It is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut.¹ CCL13 activates monocytes, but does not induce their chemotaxis. CCL14 is of recent interest because of its natural ability to bind to and signal through the human immunodeficiency virus type-1 (HIV-1) co-receptor, CC chemokine receptor 5 (CCR5).²

The biological activity of Human HCC-1 was determined by its ability to chemoattract cultured human monocytes or BaF/3 hCCR1 transfected cells. The expected ED₅₀ for these effects are typically 5 - 15 μg/ml or 0.2 - 0.8 μg/ml, respectively.

tktesssrgp yhpseccfty ttykiprqri mdyyetnsqc skpgivfitk rghsvctnps dkwvqdyikd mken

Thr20

Lyophilized

The predicted molecular weight of Recombinant Human CCL14 is Mr 8.7 kDa.

8.7

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Schulz-Knappe, P. et al. (1996) J Exp Med. 183: 295
2. Choe, S. et al. (2007) Biochemistry 46: 10008