Recombinant Human CCL21 (Mucin)
BackgroundChemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). CCL21 elicits its effects by binding to a cell surface chemokine receptor known as CCR7. In the cancer biology CCL21 seems to have multifaceted roles. CCL21 attracts CCR7 bearing cells especially T and dendritic cells but also various cancer cells. Besides the antitumour role as leukocyte recruiting, CCL21 has been shown to facilitate dendritic cell functions and to exert an angiostatic effect. Human and mouse 6Ckine are highly conserved, exhibiting 86% amino acid sequence identity. Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <1.0 EU/µg as determined by the LAL method Fusion Protein Tag Histidine Tag Protein Accession No. NP_002980 & P78423 Amino Acid Sequence (Human 6Ckine) sdggaqdccl kysqrkipak vvrsyrkqep slgcsipail flprkrsqae lcadpkelwv qqlmqhldkt pspqkpaqgc rkdrgasktg kkgkgskgck rtersqtpkg pgs, (Human Fractalkine mucin-like stalk) fekqigevkp rttpaaggmd esvvlepeat gesssleptp ssqeaqralg tspelptgvt gssgtrlppt pkaqdggpvg telfrvppvs taatwqssap hqpgpslwae aktseapstq dpstqastas spapeenaps egqrvwgqgq sprpensler eemgpvpaht dafqdwgpgs mahvsvvpvs segtpsrepv asgswtpkae epihatmdpq rlgvlitpvp daqaatrrqk qnhhhhhh N-terminal Sequence Analysis Ser24 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human 6Ckine is Mr 38.3 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 95-105 kDa. Predicted Molecular Mass 38.3 Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient NCBI Gene Bank Leinco Protein AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Recombinant Human CCL21 (Mucin) is a valuable tool for research applications due to its multifaceted biological functions and well-characterized properties as a chemokine with significant immunological relevance. Immune Cell Recruitment and ActivationCCL21 functions as a potent chemoattractant for specific immune cell populations, making it essential for studies involving immune cell trafficking and activation. The protein selectively stimulates chemotaxis of T lymphocytes and dendritic cells through binding to the CCR7 receptor. This chemotactic capability is particularly valuable because it facilitates the co-localization of antigen-stimulated dendritic cells and naïve T cells, allowing for T cell activation and induction of immune responses. The protein demonstrates an ED50 of 0.5-1.5 µg/mL for chemoattraction of human peripheral blood lymphocytes, providing quantifiable bioactivity for experimental design. Dendritic Cell EnhancementBeyond recruitment, CCL21 enhances dendritic cell functionality in multiple ways. Research demonstrates that the protein increases apoptotic resistance and endocytic capabilities of dendritic cells, while also improving their survival and cytotoxic activity. These properties make it particularly useful for studies investigating dendritic cell biology and their role in immune responses. Cancer Immunotherapy ApplicationsCCL21 has emerged as a significant candidate for cancer immunotherapy research. The protein exhibits antitumor activity through leukocyte recruitment and can inhibit immunosuppressive neutrophil polarization by suppressing NF-κB pathway activation. Studies show that CCL21 enhances the therapeutic efficacy of immune checkpoint inhibitors, suggesting potential for combination immunotherapy approaches. Additionally, the protein exerts angiostatic effects, which may contribute to tumor growth inhibition. Technical AdvantagesThe mucin-stalk chimera variant offers practical advantages for research. The protein is typically supplied as a lyophilized preparation with high purity (>95% by SDS-PAGE) and low endotoxin levels (<1.0 EU/µg). It demonstrates stability for 6-12 months when stored desiccated at -20°C to -70°C, and can be reconstituted for short-term storage at 2-8°C or long-term storage at -20°C to -70°C. The mucin-stalk chimera formulation has been specifically validated for bioactivity assays. Translational Research PotentialThe protein's effectiveness can be further enhanced through nanoformulation approaches, which enable sustained release and improved delivery to internal tumor sites. This opens possibilities for developing advanced delivery systems and studying prolonged immunological effects in complex tissue environments. Human and mouse CCL21 sequences exhibit 86% amino acid identity, facilitating translational research between in vitro human studies and in vivo murine models. You can use recombinant human CCL21 (mucin) as a standard for quantification or calibration in ELISA assays, provided it is of high purity and its concentration is accurately determined. Key considerations:
Best practices:
Summary: Recombinant Human CCL21 (Mucin) has been validated primarily for bioactivity assays, especially for its chemotactic effects on lymphocytes, and has been widely used in research on immune cell migration, tumor immunology, and dendritic cell function. Key validated applications in published research include:
Additional research applications include:
Summary Table: Validated Applications
Note: Most published research uses recombinant CCL21 (including mucin stalk chimeras) for mechanistic studies in immunology, cancer biology, and cell migration. The protein is typically validated for chemotactic bioactivity, and its use in animal models and in vitro systems is well documented. To reconstitute and prepare Recombinant Human CCL21 (Mucin) protein for cell culture experiments, follow these steps:
Summary Table: Reconstitution and Storage
These guidelines are consistent with best practices for recombinant chemokine handling and are suitable for cell culture applications. Certificate of AnalysisIMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
