Recombinant Human CNTF

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Pricing & Details

Product. No.C387
Alternate Names
HCNTF
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
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C387-10 µg
10 µg
$219.00
In stock
Max:
Min: 1
Step: 1
C387-50 µg
50 µg
$659.00
In stock
Max:
Min: 1
Step: 1

Data

Background

Ciliary neurotrophic factor (CNTF), also known as HCNTF, is a cytokine that supports survival and/or differentiation of a variety of neuronal cell types including sensory, sympathetic and motoneurons (1). It is structurally related to IL-6, IL-11, LIF and OSM. CNTF is expressed in glial cells within the central and peripheral nervous systems. It lacks the classical signal peptide sequence of a secreted protein, but is thought to convey its cytoprotective effects after release by some mechanism induced by injury. The receptors for CNTF are CNTF Rα subunit and gp130, which are expressed exclusively in the nervous system and skeletal muscle. In addition to its neuronal actions, CNTF also acts on non-neuronal cells such as glia, hepatocytes, skeletal muscle, embryonic stem cells and bone marrow stromal cells. Because CNTF is predominantly expressed in the nervous system, its modulation may have implications for Parkinson's disease, ALS and cell-replacement therapies for other disorders (2-3). CNTF mimics the biological actions of leptin while overcoming "leptin resistance" and appears to reduce inflammatory signaling cascades associated with lipid accumulation in liver and skeletal muscle (4). Therefore, it may be a therapeutic target for obesity-related metabolic disease. CNTF is highly conserved across species and exhibits cross-species bioactivity.

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CNTF was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323). The expected ED50 is typically 50 - 150 ng/ml.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
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N-terminal Sequence Analysis
Ala2
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CNTF is Mr 22.8 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 23-26 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) and cysteine pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
The lyophilized protein should be stored desiccated at -20°C. The reconstituted protein can be stored for at least one week at 4°C. For long-term storage of the reconstituted protein, aliquot into working volumes and store at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Thoenen, H. et al. (1994) J. Neurobiol. 25:1436
2. Hagg, T. et al. (2008) J. Neurosci. 28:2231
3. Masu, Y. et al. (1993) Nature 365:27
4. Febbraio, MA. et al. (2008) J. Mol. Med. 86:3
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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