Recombinant Human E-Cadherin

Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. ^{1, 2} The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma. ³

The biological activity of Human E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7human breast adenocarcinoma cells. When 5 x 10⁴ cells/well are added to rhECadherin coated plates (1.5 μg/ml with 100 μl/well), approximately 60-80% will adhere after 90 minutes at room temperature.

HEK293-derived human E-Cadherin protein Asp155-Ile707, with a C-terminal 6-His tag

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Asp155

Lyophilized

The predicted molecular weight of Recombinant Human E-Cadherin is Mr 61 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 74-92 kDa.

87.7

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Christofori, G. et al.(1998) Am. J. Hum. Genet. 63: 1588
2. Gumbiner, BM. et al.(2003) J. Cell Biol.161: 1191
3. Isaacs, WB. et al.(1995) World J Urol.13:364