Recombinant Mouse E-Cadherin

Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. ^{1, 2} The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma. ³

The biological activity of Mouse E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7 human breast adenocarcinoma cells. When 5 x 10⁴ cells/well are added to rmECAD coated plates (1.5 μg/ml with 100 μl/well), approximately 50-70% will adhere after 90 minutes at room temperature.

Fc Fusion Protein

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Asp157

Lyophilized

The predicted molecular weight of Recombinant Mouse ECAD is Mr 62 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 75-87 kDa.

62

This recombinant protein was 0.2 µm filtered and lyophilized from a sterile solution containing 50mM Tris-Citrate, 50 mM NaCl and 2 mM CaCl₂, pH 6.5.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Christofori, G. et al. (1998) Am. J. Hum. Genet. 63: 1588
2. Gumbiner, BM. et al. (2003) J. Cell Biol.161: 1191
3. Isaacs, WB. et al. (1995) World J Urol. 13:364