Recombinant Human E-Cadherin

Recombinant Human E-Cadherin

Product No.: C1349

[product_table name="All Top" skus="C1349"]

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Alternate Names
Cell-CAM120/80, Uvomorulin, Arc-1, L-CAM CDH1, CD324, CDHE, UVO
Product Type
Recombinant Protein
Expression Host
HEK-293 Cells
Species
Human
Applications
ELISA Cap

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Select Product Size
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Background

Cadherin 1, type 1, E-cadherin (epithelial), also known as CDH1 and CD32, is a tumor suppressor gene. 1, 2 The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. E-cadherin-mediated cell-cell adhesion pathway may represent a novel chemotherapeutic target for bladder cancer, prostate cancer, and renal-cell carcinoma. 3

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human E-CAD was determined by the ability of the immobilized protein to support the adhesion of the MCF7human breast adenocarcinoma cells. When 5 x 10<sup>4</sup> cells/well are added to rhECadherin coated plates (1.5 μg/ml with 100 μl/well), approximately 60-80% will adhere after 90 minutes at room temperature.
Fusion Protein Tag
HEK293-derived human E-Cadherin protein Asp155-Ile707, with a C-terminal 6-His tag
Protein Accession No.
Amino Acid Sequence
dwvipp iscpenekgp fpknlvqiks nkdkegkvfy sitgqgadtp pvgvfiiere tgwlkvtepl dreriatytl fshavssngn avedpmeili tvtdqndnkp eftqevfkgs vmegalpgts vmevtatdad ddvntynaai aytilsqdpe lpdknmftin rntgvisvvt tgldresfpt ytlvvqaadl qgeglsttat avitvtdtnd nppifnptty kgqvpenean vvittlkvtd adapntpawe avytilnddg gqfvvttnpv nndgilktak gldfeakqqy ilhvavtnvv pfevslttst atvtvdvldv neapifvppe krvevsedfg vgqeitsyta qepdtfmeqk ityriwrdta nwleinpdtg aistraeldr edfehvknst ytaliiatdn gspvatgtgt lllilsdvnd napipeprti ffcernpkpq viniidadlp pntspftael thgasanwti qyndptqesi ilkpkmalev gdykinlklm dnqnkdqvtt levsvcdceg aagvcrkaqp veaglqiieg rmdpkscdkt htcppcpape llggpsvflf ppkpkdtlmi srtpevtcvv vdvshedpev kfnwyvdgve vhnaktkpre eqynstyrvv svltvlhqdw lngkeykckv snkalpapie ktiskakgqp repqvytlpp srdeltknqv sltclvkgfy psdiavewes ngqpennykt tppvldsdgs fflyskltvd ksrwqqgnvf scsvmhealh nhytqkslsl spgkhhhhhh
N-terminal Sequence Analysis
Asp155
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human E-Cadherin is Mr 61 kDa. However, the actual molecular weight as observed by migration on SDS Page is Mr 74-92 kDa.
Predicted Molecular Mass
87.7
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Christofori, G. et al.(1998) Am. J. Hum. Genet. 63: 1588
2. Gumbiner, BM. et al.(2003) J. Cell Biol.161: 1191
3. Isaacs, WB. et al.(1995) World J Urol.13:364
Elisa Sandwich Protocol
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.