Recombinant Human EDA-A2

Ectodysplasin A (EDA) is a ligand of the tumor necrosis factor (TNF) family that has been shown to play a crucial role in ectodermal differentiation. EDA-A1 and EDA-A2 are the two main splice variants of EDA. ¹ EDA-A1 binds only the receptor EDAR, whereas EDA-A2 binds only the related, but distinct, X-linked ectodysplasin-A2 receptor (XEDAR). EDA-A1 and EDA-A2 are differentially expressed and play a role in epidermal morphogenesis.
EDA-A2 is a type III integral membrane protein with three CYS region at the N terminal region. ² EDA-A2 may be a promising agent for the gene therapy of osteosarcoma.

Histidine Tag

mhhhhhhhhh hgggsgggsg ggsiegragp pgpngppgpp gppgpqgppg ipgipgipgt tvmgppgppg ppgpqgppgl qgpsgaadka gtrenqpavv hlqgqgsaiq vkndlsggvl ndwsritmnp kvfklhprsg elevlvdgty fiysqvyyin ftdfasyevv vdekpflqct rsietgktny ntcytagvcl lkarqkiavk mvhadisinm skhttffgai rlgeapas

Met

Lyophilized

The predicted molecular weight of Recombinant Human EDA-A2 is Mr 24.7 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 28-38 kDa.

24.7

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.4 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Chaudhary, PM. et al.(2004)Protein Expr Purif 37:162
2. Dixit, VM. et al.(2000)Science 290:523