Fibroblast growth factor 16 (FGF-16) is a member of the fibroblast growth factor (FGF) family, which are all heparin-binding growth factors. FGF-16 is most similar to FGF-9 sharing 73% amino acid identity. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-16 is released from neonatal cardiac myocytes and alters growth-related signaling, possibly interfering with cardiac myocyte proliferative potential (2). It lacks a typical signal peptide but is efficiently generated by mechanisms other than the classical protein secretion pathway. FGF-16 signals through the receptors FGFR 2c and 3c (3). It induces proliferation of primary adipocytes and oligodendrocytes in vitro and stimulates liver weight increase in vivo (4).
>97% by SDS-PAGE and analyzed by silver stain.
<0.01EU/µg as determined by the LAL method
The biological activity of Human FGF-16 was determined by its ability stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Rizzino, A. et al., 1988, Cancer Research 48:4266 - 4271; Thomas, K., 1987, in Methods in Enzymology 147:120 - 135). The expected ED50 for this effect is typically 7.5 - 30 ng/mL.
Protein Accession No.
Amino Acid Sequence
aevggvfas ldwdlhgfss slgnvplads pgflnerlgq iegklqrgsp tdfahlkgil rrrqlycrtg fhleifpngt vhgtrhdhsr fgilefisla vglisirgvd sglylgmner gelygskklt recvfreqfe enwyntyast lykhsdserq yyvalnkdgs pregyrtkrh qkfthflprp vdpsklpsms rdlfhyr
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human FGF-16 is Mr 23.7 kDa.
This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, EDTA, Dithiothreitol (DTT), and sodium sulphate (Na2SO4).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
Next Day Ambient
NCBI Gene Bank
References & Citations
1. Cattini, PA. et al. (2003) Mol. Cell. Biochem. 242:65
2. Lu, SY. et al. (2008) Amer. J. Physiol. 294:1242
3. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694
4. Konishi, M. et al. (2000) J. Biol. Chem. 275:12119
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.