BackgroundFibroblast growth factor 16 (FGF-16) is a member of the fibroblast growth factor (FGF) family, which are all heparin-binding growth factors. FGF-16 is most similar to FGF-9 sharing 73% amino acid identity. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-16 is released from neonatal cardiac myocytes and alters growth-related signaling, possibly interfering with cardiac myocyte proliferative potential (2). It lacks a typical signal peptide but is efficiently generated by mechanisms other than the classical protein secretion pathway. FGF-16 signals through the receptors FGFR 2c and 3c (3). It induces proliferation of primary adipocytes and oligodendrocytes in vitro and stimulates liver weight increase in vivo (4). Protein DetailsPurity >97% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.01EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF-16 was determined by its ability stimulate 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts
(Rizzino, A. et al., 1988, Cancer Research 48:4266 - 4271; Thomas, K., 1987, in Methods in Enzymology 147:120 - 135). The expected ED50 for this effect is typically 7.5 - 30 ng/mL. Expression Host E. coli Protein Accession No. Amino Acid Sequence aevggvfas ldwdlhgfss slgnvplads pgflnerlgq iegklqrgsp tdfahlkgil rrrqlycrtg fhleifpngt vhgtrhdhsr fgilefisla vglisirgvd sglylgmner gelygskklt recvfreqfe enwyntyast lykhsdserq yyvalnkdgs pregyrtkrh qkfthflprp vdpsklpsms rdlfhyr
N-terminal Sequence Analysis Ala2 State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF-16 is Mr 23.7 kDa. Formulation This recombinant protein was lyophilized from a 0.2 μm filtered solution in MOPS, EDTA, Dithiothreitol (DTT), and sodium sulphate (Na2SO4). Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient PubMed NCBI Gene Bank References & Citations1. Cattini, PA. et al. (2003) Mol. Cell. Biochem. 242:65 2. Lu, SY. et al. (2008) Amer. J. Physiol. 294:1242 3. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694 4. Konishi, M. et al. (2000) J. Biol. Chem. 275:12119 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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