BackgroundFibroblast growth factor 17, also known as FGF17 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes including embryonic development cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-17 is most similar to FGF-8 and is highly conserved between mouse and human (93 % identity).1,2 FGF17 is required for several complex social behaviors and studies suggest that disturbances in FGF17 signaling may contribute to neuropsychiatric diseases that affect such behaviors.3 Protein DetailsPurity >95% by SDS-PAGE and analyzed by silver stain. Endotoxin Level <0.1 EU/µg as determined by the LAL method Biological Activity The biological activity of Human FGF-17 was determined by its ability to stimulate 3H-thymidine incorporation by NR6R-3T3 fibroblast. The expected ED50 for this effect is typically 15 - 60 ng/ml, in the presence of 1 μg/ml heparin. Expression Host E. coli Protein Accession No. Amino Acid Sequence tqgenhpspn fnqyvrdqga mtdqlsrrqi reyqlysrts gkhvqvtgrr isataedgnk faklivetdt fgsrvrikga esekyicmnk rgkligkpsg kskdcvftei vlennytafq narhegwfma ftrqgrprqa srsrqnqrea hfikrlyqgq lpfpnhaekq kqfefvgsap trrtkrtrrp qplt N-terminal Sequence Analysis Thr23 (with and without an N-terminal Met) State of Matter Lyophilized Predicted Molecular Mass The predicted molecular weight of Recombinant Human FGF-17 is Mr 22.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is 21.5 and 22.6 kDa (reducing conditions). Formulation This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives. Storage and Stability This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions. Country of Origin USA Shipping Next Day Ambient PubMed NCBI Gene Bank References & Citations1. Hoshikawa, M. et al. (1998) Biochemical and Biophysical Research Communications 244: 187 2. Xu, J. et al. (1999) Mechanisms of Development 83: 165 3. Mucke, L. et al. (2008) Genes Brain Behav. 7: 344 IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein. |
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