Recombinant Human FGF-4

Fibroblast growth factor 4 (heparin secretory transforming protein 1, Kaposi sarcoma oncogene), also known as FGF4 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF-4 has an ability to promote neural stem cell proliferation and neuronal differentiation in the postnatal brain.¹

The biological activity of Human FGF-4 was monitored in a mitogenic assay by measuring the FGF dependent 3H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts (Thomas, K., 1987 Methods in Enzymology 147:120 - 135; Rizzino, A. et al., 1988, Cancer Res. 48:4266). The expected ED₅₀ for this effect is typically 0.05 - 0.15 ng/mL.

The DNA sequence encoding the putative mature 176 amino acid residue FGF-4 (excluding the 30 amino acid residue deduced signal peptide) was cloned into a suitable expression vector and expressed in E. coli (Delli Bovi, P. et al., 1987, Cell 50:729 - 737; Miyagawa, K. et al., 1988, Oncogene 3:383 - 389).

Lyophilized

This preparation of Recombinant Human FGF-4 is subject to proteolytic cleavage, which results in a mixture of 4 peptides and reveals bands on an SDS PAGE gel at Mr 14 kDa & 16 kDa.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Ochiya T. et al. (2006) FASEB J. 20: 1848