Recombinant Human Follistatin

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Pricing & Details

Product. No.F168
Alternate Names
FS, Activins-Binding Protein, FSH-Suppressing Protein
Expression Host
Sf 21 Insect Cells
Species
Human
Prod No.
Size
Price
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F168-25 µg
25 µg
$359.00
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Step: 1

Background

Follistatin (FST) is a glycosylated single-chain protein. It specifically inhibits the secretion of FSH from the pituitary.1 Follistatin/activin-binding protein binds to heparan sulfate side chains of proteoglycans on the granulosa cell surface to regulate the various actions of activin.2 Follistatin regulates a variety of biological functions, including cell proliferation, differentiation, and apoptosis. Follistatin plays a critical role in the production of multiple-organ metastasis, predominantly by inhibiting the angiogenesis.3

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.01EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human FS was determined by its ability to neutralize Activin-induced bioactivity on K562 cells (erythroid differentiation). The expected ED50 for this effect is typically 0.1 - 0.4 μg/ml in the presence of 7.5 ng/ml of rhActivin A.
Expression Host
Sf 21 Insect Cells
Protein Accession No.
Amino Acid Sequence
mg ncwlrqakng rcqvlyktel skeeccstgr lstswteedv ndntlfkwmi fnggapncip cketcenvdc gpgkkcrmnk knkprcvcap dcsnitwkgp vcgldgktyr necallkarc keqpelevqy qgrckktcrd vfcpgsstcv vdqtnnaycv tcnricpepa sseqylcgnd gvtyssachl rkatcllgrs iglayegkci kakscediqc tggkkclwdf kvgrgrcslc delcpdsksd epvcasdnat yasecamkea acssgvllev khsgscnsis edteeeeed
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human FS is Mr 31 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 40 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Ling, N. et al. (1989) Mol Endocrinol. 3: 651
2. Sugino, H. et al. (1991) J Biol Chem. 266: 19432
3. Sone, S. et al. (2008) Clin Cancer Res. 14: 660
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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